O-CAS, a fast and universal method for siderophore detection

Pérez-Miranda, Sandra; Cabirol, Nathalie; George-Téllez, R.; Zamudio‐Rivera, Luis S.; Fernández, Francisco

doi:10.1016/j.mimet.2007.03.023

Cited by 324 publications

(230 citation statements)

References 21 publications

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“…A number of modified CAS assays have been developed to allow for the growth of these organism (1,5,7). One of the easiest is the O-CAS method, where different microorganisms can be grown on their optimal media and then overlaid with CAS agar (7).…”

Section: Resultsmentioning

confidence: 99%

Use of Blue Agar CAS Assay for Siderophore Detection

Louden

Haarmann

Lynne

2011

J Microbiol Biol Educ.

393

180

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Schwyn and Neiland developed a universal siderophore assay using chrome azurol S(CAS) and hexadecyltrimethylammonium bromide (HDTMA) as indicators. In Schwyn and Neiland’s original paper, the procedure given for making CAS agar is written in general terms and can be difficult to follow, especially for an individual who has limited experience making more complex media. Here, we give a step-by-step protocol for making the CAS agar plates, and we discuss how this media can be incorporated in a comprehensive project in a microbiology lab course for biology majors

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Section: Resultsmentioning

confidence: 99%

Use of Blue Agar CAS Assay for Siderophore Detection

Louden

Haarmann

Lynne

2011

J Microbiol Biol Educ.

393

180

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“…The analysis for the presence of various bioactive and inactive GAs in the culture extract was undertaken according to the Lee et al (1998). The concentrations of different types of GAs were calculated in ng ml enhanced by siderophores produced by several microbes (Miethke & Marahiel 2007;Pérez-Miranda et al 2007). Photorhabdus spp.…”

Section: Siderophore Production Assaymentioning

confidence: 99%

Gibberellins synthesized by the entomopathogenic bacterium, Photorhabdus temperata M1021 as one of the factors of rice plant growth promotion

Ullah

Khan

Jung

et al. 2014

Journal of Plant Interactions

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In the present study, different types of gibberellins (GAs) in the culture filtrate (CF) of Photorhabdus temperata M1021 were quantified. The analysis of CF helped in profiling various bioactive GAs: GA 1 , GA 3 , GA 4 , and GA 7 . Several physiologically inactive GAs: GA 9 , GA 12 , and GA 20 were detected as well. Siderophore production was also investigated by growing P. temperata M1021 on chrome azurol-S blue agar plates. Furthermore, the strain was inoculated into 'Waito-C' (Oryza sativa L.) rice plants, which significantly (P < 0.05) increased plant growth attributes such as plant length, chlorophyll content, and fresh and dry biomass compared with those in controls. In a separate experiment, canola (Brassica napus L.) seeds treated with CF of M1021 were significantly (P < 0.05) accelerated germination rate as well as biomass production. Findings of the present study suggest that the strain M1021 contributes an important role in the plant growth by synthesizing a wide array of bioactive metabolites.

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“…Solubilization of mineral phosphate is characterized by the formation of a clear halo around bacterial colonies with phosphate solubilization capacity. The O-CAS assay was used to detect siderophore produced by antagonistic endophytic bacteria (Perez-Miranda et al, 2007). Pure bacteria were stabbed on CAS agar plates and incubated at 28 o C for 2-3 days.…”

Section: Methodsmentioning

confidence: 99%