O-198. Pregnancies after vitrification of human day 5 embryos

Vanderzwalmen, Pierre; Delval, A.; Chatziparasidou, A.; Bertin, G.; Ectors, Fabien; Lejeune, Bernard

doi:10.1093/humrep/12.suppl_2.98

Cited by 17 publications

(11 citation statements)

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“…Les premiers succès ont été obtenus en utilisant la minipaillette (250 µl) comme méthode de stockage des embryons de souris [2,4], bovins [5] et humain [6]. L'épaisseur de la paroi de la paillette, le volume de liquide dans celle-ci (250 µl) et la formation d'un isolant thermique (effet Leidenfrost) autour de la paillette lors de l'immersion dans l'azote liquide ne permettaient pas d'atteindre des vitesses de refroidissement supérieures à 2 000°C/min.…”

Section: Deuxième Condition : Vitesse De Refroidissement Et Supports unclassified

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Cryopréservation des embryons humains par vitrification

Vanderzwalmen

Zech

Greindl

et al. 2006

Gynécologie Obstétrique & Fertilité

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Section: Deuxième Condition : Vitesse De Refroidissement Et Supports unclassified

“…En 1995, la mise sur le marché des milieux séquentiels nous a incité à effectuer des cultures prolongées jusqu'au stade de blastocyste et c'est en 1996 que nous avons obtenu nos premiè-res grossesses après vitrification de jeunes blastocystes [6].…”

Section: Pourquoi Certains Blastocystes Sont-ils Rebelles à La Cryocounclassified

Cryopréservation des embryons humains par vitrification

Vanderzwalmen

Zech

Greindl

et al. 2006

Gynécologie Obstétrique & Fertilité

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“…Recently, the movement towards transferring fewer embryos has resulted in greater numbers of surplus embryos, leading to a greater likelihood of having embryos cryopreserved [ 3 ]. Other applications of cryopreservation include cases where severe ovarian hyperstimulation syndrome is anticipated, various situations where fertilization is not possible on the day of oocyte retrieval, a discrepancy between the oocyte donor's oocyte retrieval period and the recipient's embryo transfer period; and cryopreservation of gametes and embryos for fertility preservation in cancer patients scheduled to receive cytotoxic chemotherapy or radiotherapy.…”

Section: Introductionmentioning

confidence: 99%

Effects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development

Kim

Park²,

Yang

et al. 2017

Clin Exp Reprod Med

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ObjectiveThis study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers.MethodsFirst, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinning-assisted hatching before vitrification (4, 6, and 8 minutes or 4, 7, and 10 minutes, respectively). After incubation, double-immunofluorescence staining was performed.ResultsThe hatched blastocyst rate 72 hours after the 2-cell embryos were thawed was significantly higher in the 2LAH-ES8 group (33.3%) than in the other groups (p<0.05). In the control-8 group (22.1±4.6), the cell number of the inner cell mass was higher than in the LAH groups (p<0.05). The number of trophectoderm cells was higher in the 2LAH-ES6 group (92.8±8.9) than in the others (p<0.05). The hatched blastocyst rate 48 hours after the 8-cell embryos were thawed was higher in the 8LAH-ES4 group (45.5%) than in the other groups, but not significantly. The inner cell mass cell number was highest in the 8LAH-ES7 group (19.5±5.1, p<0.05). The number of trophectoderm cells was higher in the 8LAH-ES10 group (73.2±12.1) than in the other groups, but without statistical significance.ConclusionWhen LAH was performed, 2-cell embryos with large blastomeres had a lower hatched blastocyst rate when the exposure to vitrification solution was shorter. Conversely, 8-cell embryos with small blastomere had a higher hatched blastocyst rate when the exposure to vitrification solution was shorter.

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“…During slow freezing, crystallization occurs abundantly, with resulting relatively low blastocyst preservation. To overcome these problems, vitrification is now applied to freeze blastocysts [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cohen et al [ 7 ] first reported the use of slow freezing cryopreservation of human blastocysts. Vitrification for human blastocysts was introduced by Vanderzwalmen et al [ 12 ] in 1992. Initially, the survival rate of frozen-thawed human blastocysts was also very disappointing.…”

Section: Introductionmentioning

confidence: 99%

Vitrification solution without sucrose for cryopreservation in mouse blastocysts

Joo

Lee

Jeong

et al. 2014

Clin Exp Reprod Med

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ObjectiveThis study was designed to investigate the survival rate of vitrified mouse blastocysts depending on the presence or absence of sucrose in vitrification solution.MethodsMouse two-cell embryos were collected and cultured to blastocysts. Two vitrification solutions were prepared. The control solution was composed of 25% glycerol, 25% ethylene glycol, and 0.5 M sucrose (G25E250.5S) containing 2.5 mL glycerol, 2.5 mL ethylene glycol, 2 mL SSS, and 0.855 g sucrose in 5 mL PB1. The experimental solution was composed of 25% glycerol and 25% ethylene glycol (G25E25) and contained 2.5 mL glycerol and 2.5 mL ethylene glycol in 5 mL PB1. Artificial shrinkage was conducted by aspirating the blastocoelic fluid using an ICSI pipette. To examine the effect of sucrose in the vitrification solution on the survival rate of mouse blastocysts, the shrunken-equilibrated blastocysts were rehydrated or vitrified after being exposed to one of the two vitrification solutions. After exposure and the vitrification-thawing process, the re-expansion rate and hatching rate were evaluated after 6 hours of in vitro culture.ResultsThe re-expansion rate of mouse blastocysts exposed to vitrification solution with and without sucrose were not different in the experimental solution (without sucrose) (98%) and the control solution (with sucrose) (92%) (p>0.05). The hatching rate was higher in the experimental solution (95%) than in the control solution (88%), but did not differ across two treatments (p>0.05). The re-expansion rate of mouse blastocysts vitrified in the control solution was 92% and 94%, respectively (p>0.05), and the hatching rate was higher in the experimental solution (90%) than in the control solution (74%) (p<0.05).ConclusionSucrose need not be added in vitrification solution for freezing of artificially shrunken mouse blastocysts.

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O-198. Pregnancies after vitrification of human day 5 embryos

Cited by 17 publications

References 0 publications

Cryopréservation des embryons humains par vitrification

Cryopréservation des embryons humains par vitrification

Effects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development

Vitrification solution without sucrose for cryopreservation in mouse blastocysts

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