(−)-Nyasol, isolated from <i>Anemarrhena asphodeloides</i> suppresses neuroinflammatory response through the inhibition of I-κBα degradation in LPS-stimulated BV-2 microglial cells

Lee, Hwa Jin; Li, Hua; Chang, Hee Ryun; Jung, Hyeyoun; Lee, Da Yeon; Ryu, Jae‐Ha

doi:10.3109/14756366.2012.697057

Cited by 9 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AA13 inhibited LPS-stimulated PGE 2 production in RAW264.7 cells Studies have found that the extracts of Anemarrhenae asphodeloides inhibited LPS-stimulated PGE 2 production in microglia [30] . To determine the effects of AA13 on the production of PGE 2 , RAW264.7 cells were pretreated with AA13 for 30 min, followed by LPS (1 μg/mL) stimulation for 24 h without removing the AA13.…”

Section: Resultsmentioning

confidence: 96%

Sarsasapogenin-AA13 inhibits LPS-induced inflammatory responses in macrophage cells in vitro and relieves dimethylbenzene-induced ear edema in mice

et al. 2017

View full text Add to dashboard Cite

Sarsasapogenin-AA13 (AA13) is a novel synthetic derivative of sarsasapogenin extracted from the Chinese herb Rhizoma Anemarrhenae. In this study we investigated the effects of AA13 on lipopolysaccharide (LPS)-induced production of inflammatory factors in macrophage cells and the anti-inflammatory activity of AA13 in an inflammatory model of dimethylbenzene-induced ear edema. Macrophage cells (RAW264.7 cells and mouse peritoneal macrophages) were exposed to LPS (1 μg/mL); pretreatment with AA13 (5-20 μmol/L) dose-dependently inhibited LPS-induced production of NO, TNF-α and PGE, and LPS-stimulated expression levels of COX-2 and iNOS. Furthermore, pretreatment with AA13 dose-dependently suppressed LPS-stimulated phosphorylation of p38 and JNK, but had no effect on ERK in RAW264.7 cells. Moreover, pretreatment with AA13 inhibited LPS-induced activation of the nuclear factor (NF)-κB in RAW264.7 cells. The in vivo anti-inflammatory activity of AA13 was demonstrated in a mouse inflammatory model: pre-treatment with either AA13 (20 mg·kg·d, ig) or a positive control antifani (10 mg·kg·d, ig) for 3 d significantly relieved dimethylbenzene-induced ear edema. Our results demonstrate that AA13 effectively inhibit LPS-induced inflammatory responses in macrophage cells in vitro and relieve dimethylbenzene-induced ear edema in vivo.

show abstract

Section: Resultsmentioning

confidence: 96%

Sarsasapogenin-AA13 inhibits LPS-induced inflammatory responses in macrophage cells in vitro and relieves dimethylbenzene-induced ear edema in mice

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Compared with QR inducing assay, NO inhibitory effect assay and other in vitro and in vivo anti-inflammatory models are classical and commonly adopted biological research methods, and accordingly more literatures concerning inflammation of TCMs have been published. Based on our findings, we carried out a systemic search of reported inflammation-related literatures of our observed 55 active TCM extracts, and concluded that: some TCMs have been comprehensively investigated for their anti-inflammatory property and resulted in the discovery of diverse types of natural products, covering agrimonolide from Agrimonia pilosa (8) [ 24 ], (-)-nyasol from Anemarrhena asphodeloides (17) [ 25 ], alantolactone from Aucklandia lappa (31) [ 26 ], berberine from Coptis chinensis (53) [ 27 ], forsythiaside from Forsythia suspensa (79) [ 28 ], resveratrol from Polygonum cuspidatum (141) [ 29 ], etc. Beside these comprehensively investigated molecules, a great deal of constituents have been isolated from these active TCMs, and required further confirmation of their anti-inflammatory function.…”

Section: Resultsmentioning

confidence: 99%

Screening of traditional Chinese medicines with therapeutic potential on chronic obstructive pulmonary disease through inhibiting oxidative stress and inflammatory response

Zhou

Xuan

et al. 2016

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundChronic obstructive pulmonary disease (COPD) is a major public health problem and gives arise to severe chronic morbidity and mortality in the world. Inflammatory response and oxidative stress play dominant roles in the pathological mechanism of COPD, and have been regarded to be two important targets for the COPD therapy. Traditional Chinese medicines (TCMs) possess satisfying curative effects on COPD under guidance of the TCM theory in China, and merit in-depth investigations as a resource of lead compounds.MethodsOne hundred ninety-six of TCMs were collected, and extracted to establish a TCM extract library, and then further evaluated for their potency on inhibitions of oxidative stress and inflammatory response using NADP(H):quinone oxidoreductase (QR) assay and nitric oxide (NO) production assay, respectively.ResultsOur investigation observed that 38 of the tested TCM extracts induced QR activity in hepa 1c1c7 murine hepatoma cells, and 55 of them inhibited NO production in RAW 264.7 murine macrophages at the tested concentrations. Noteworthily, 20 of TCM extracts simultaneously inhibited oxidative stress and inflammatory responses.ConclusionThe observed bioactive TCMs, particularly these 20 TCMs with dual inhibitory effects, might be useful for the treatment of COPD. More importantly, the results of the present research afford us an opportunity to discover new lead molecules as COPD therapeutic agents from these active TCMs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1347-y) contains supplementary material, which is available to authorized users.

show abstract

“…Nyasol was isolated from A. cochinchinensis many years ago, but there are few reports on its pharmacological properties [ 42 ]. Nyasol has demonstrated anti-inflammatory effects in LPS-activated BV-2 microglial cells [ 43 ]. In our study, we found that UNPD43533 (Nyasol) targeted 11-DH, GR, and PPAR γ ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Asparagus cochinchinensis Extract Alleviates Metal Ion‐Induced Gut Injury in Drosophila: An In Silico Analysis of Potential Active Constituents

Wei-yu

Jin

2016

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Metal ions and sulfate are components of atmospheric pollutants that have diverse ways of entering the human body. We used Drosophila as a model to investigate the effect of Asparagus cochinchinensis (A. cochinchinensis) extracts on the gut and characterized gut homeostasis following the ingestion of metal ions (copper, zinc, and aluminum). In this study, we found that the aqueous A. cochinchinensis extract increased the survival rate, decreased epithelial cell death, and attenuated metal ion-induced gut morphological changes in flies following chronic exposure to metal ions. In addition, we screened out, by network pharmacology, six natural products (NPs) that could serve as putative active components of A. cochinchinensis that prevented gut injury. Altogether, the results of our study provide evidence that A. cochinchinensis might be an effective phytomedicine for the treatment of metal ion-induced gut injury.

show abstract

(−)-Nyasol, isolated from Anemarrhena asphodeloides suppresses neuroinflammatory response through the inhibition of I-κBα degradation in LPS-stimulated BV-2 microglial cells

Cited by 9 publications

References 31 publications

Sarsasapogenin-AA13 inhibits LPS-induced inflammatory responses in macrophage cells in vitro and relieves dimethylbenzene-induced ear edema in mice

Sarsasapogenin-AA13 inhibits LPS-induced inflammatory responses in macrophage cells in vitro and relieves dimethylbenzene-induced ear edema in mice

Screening of traditional Chinese medicines with therapeutic potential on chronic obstructive pulmonary disease through inhibiting oxidative stress and inflammatory response

Asparagus cochinchinensis Extract Alleviates Metal Ion‐Induced Gut Injury in Drosophila: An In Silico Analysis of Potential Active Constituents

Contact Info

Product

Resources

About