Nutritive value of leaf protein concentrates. II.

Cowlishaw, S. J.; Eyles, D. E.; Raymond, W. F.; Tilley, J. M. A.

doi:10.1002/jsfa.2740071208

Cited by 17 publications

(15 citation statements)

References 5 publications

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“…The nutritive value of leaf protein concentrates from M. sativa has been investigated earlier in other laboratories with higher animals. Thus, Cowlishaw, Eyles, Raymond & Tilley (1956) and Ferrando (1969) found that this species contains toxic substances that lower the nutritive value but which can be removed by washing and heating. It is interesting that this species gave low values with the Tetrahymena test as well.…”

Section: Nutritive Valuementioning

confidence: 99%

Quantities and qualities of leaf protein concentrates from wild species and crop species grown under controlled conditions

Lexander

Carlsson

Schalén

et al. 1970

Annals of Applied Biology

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The amount of protein produced per m2 by plants cultivated in soil in a greenhouse at three levels of fertilizer application, for c. 10 weeks, was estimated by Kjeldahl analysis of the TCA-insoluble fraction of freeze-dried material. The quantity of protein extractable for production of leaf protein concentrates was determined by Kjeldahl analysis of non-dried but frozen material after disintegration with a meat mincer and an Ultra-Turrax homogenizer, hydraulic pressing and filtration, and protein precipitation by heat (in two steps) or by TCA. The precipitability was also studied by Folin-Ciocalteu determination of protein. The quality of each protein concentrate was studied by determining nitrogen content (Kjeldahl), digestibility by pepsin and by pepsin+ pancreatin, nutritive value in Tetrahymena tests, and lysine and methionine content (analysed microbiologically).Twenty-nine species and varieties were investigated. Large differences between species were found in all the properties studied. Protein extractability varied between 5 and 80%, while the extractable protein produced per m2 ranged between I and 140 g. The highest digestibility was two to three times greater than the lowest one. In the most digestible species (Amaranthus caudatus), 82% of the N of the chloroplastic protein concentrate was digested by pepsin +pancreatin. The Tetrahymena value generally ranged between 40 and 98, whereas casein gave values of about 75. The lysine content always exceeded the FA0 minimum. The methionine content of most species varied between 2.0 and 2.2% of the hydrolyzed protein.Amaranthus caudatus and the Chenopodiaceae investigated were the most suitable species for large-scale production of leaf protein concentrates for human consumption because they gave high yield of extractable protein and high-quality protein concentrates.

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Section: Nutritive Valuementioning

confidence: 99%

Quantities and qualities of leaf protein concentrates from wild species and crop species grown under controlled conditions

Lexander

Carlsson

Schalén

et al. 1970

Annals of Applied Biology

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“…This juice can either be fed directly or heated to precipitate a protein-rich coagulum, which is filtered off and fed, either wet or after drying. Results of feeding experiments with the coagulated proteins have been reported (2,3).…”

Section: Introductionmentioning

confidence: 99%

The Value of the Fibrous Residue From Leaf Protein Extraction as a Feeding‐stuff for Ruminants

Raymond

Harris

1957

Grass and Forage Science

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The production of a fibrous material as a residue from the process of extracting a protein concentrate from fresh crops is described and the value of this material as a coarse feed for ruminants has been studied in five digestibility experiments with sheep. In these the digestibilities of the residues have been compared with the digestibilities of the corresponding fresh crops. The fibre residues, both fresh and artificially dried, were readily consumed by sheep. The average digestibilities of the dry matter and organic matter in the fibre residues were respectively 6.4 units and 5.6 units lower than in the corresponding fresh crops. The organic matter content of the fibre residues was higher than that of the fresh crops. Because of this the residues contained 94% as much digestible organic matter per lb. of feed dry matter as did the unprocessed crops. The dry matter of the fibre residues contained from 52%–60% of digestible organic matter and from 1.32% to 2.2% of digestible nitrogen. In respect to the former they are equivalent to medium grade hay, but are superior to this in digestible nitrogen content. The fibre residues can be readily dried but artificial drying would be unlikely to be economic in commercial practice. Residues have been successfully ensiled, using either molasses or mineral acids as additives but the silage has been found to be quite unpalatable to all classes of ruminants. It is suggested that the fibre residues would best be utilized by feeding directly to stock in yards adjacent to the protein extraction machinery.

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“…Other data are presented on the chemical compositions of some of the fractions prepared. PROCESSING FRESH ALFALFA tO Separate nonfibrous from fibrous matter has been studied in a number of laboratories (7,4,6,8). These studies and some on other herbage (4,6) have shown that a material of high nitrogen and low fiber content can be separated by processing methods involving grinding or macerating and then squeezing or pressing the broken tissue to extrude the juice.…”

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“…PROCESSING FRESH ALFALFA tO Separate nonfibrous from fibrous matter has been studied in a number of laboratories (7,4,6,8). These studies and some on other herbage (4,6) have shown that a material of high nitrogen and low fiber content can be separated by processing methods involving grinding or macerating and then squeezing or pressing the broken tissue to extrude the juice. Heat treatment of the juice has been reported to yield an insoluble product, high in nitrogen, which with some leaf crops accounts for as much as 50 to 60% of the protein (7).…”

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confidence: 99%

Alfalfa Fractionation, Effects of Various Procedures in Laboratory Processing of Fresh Alfalfa on Separation of Nitrogen and Solids from Fiber

Halverson¹

1962

J. Agric. Food Chem.

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Nutritive value of leaf protein concentrates. II.

Cited by 17 publications

References 5 publications

Quantities and qualities of leaf protein concentrates from wild species and crop species grown under controlled conditions

Quantities and qualities of leaf protein concentrates from wild species and crop species grown under controlled conditions

The Value of the Fibrous Residue From Leaf Protein Extraction as a Feeding‐stuff for Ruminants

Alfalfa Fractionation, Effects of Various Procedures in Laboratory Processing of Fresh Alfalfa on Separation of Nitrogen and Solids from Fiber

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