Nutritive Composition and Antimicrobial Activity of Moringa stenopetala (Baker f.) Cufod

Raghavendra, HL; Kekuda, T.R.Prashith; Vijayananda, B N; Duressa, Dereje; Solomon, Tifsehit

doi:10.9734/jamps/2016/29987

Cited by 10 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The variation in the antimicrobial activities may be attributed to differences in the time of harvest (Celikel and Kavas, 2008), the developmental stage of plants (Ruberti and Baratta, 2000) and the method of extraction (Manilal and Idhayadhulla, 2014). Prior studies affirmed the antibacterial activities of M. stenopetala against S. aureus (Tesemma et al, 2013;Raghavendra et al, 2016;El-Fadl et al, 2017), however, the antibiosis against biofilm-forming MRSA has seldom been reported from Ethiopia. In comparison to our study, 80 % methanolic extract of M. stenopetala seeds showed a much lower MIC value of 125 μg/ml against S. aureus (Chekesa and Mekonnen, 2015).…”

Section: Primary Screeningmentioning

confidence: 99%

In vitro antibacterial activity of medicinal plants against biofilm-forming methicillin-resistant Staphylococcus aureus: efficacy of Moringa stenopetala and Rosmarinus officinalis extracts

Manilal

Sabu

Shewangizaw

et al. 2020

Heliyon

View full text Add to dashboard Cite

The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) is slowly rising in Ethiopia for the past few decades. Therefore, novel classes of antibiotics are indispensable to combat the increased incidence of newly emerging multidrug-resistant bacteria like MRSA. Terrestrial flora is considered as a reservoir of novel bioactive secondary metabolites as they have provided us with the largest array of natural products. In this background, the present study is intended to evaluate the in-vitro antibacterial efficacy of five medicinal plants (Ocimum lamiifolium Hochst. ex Benth., Rosmarinus officinalis L, Catharanthus roseus Linn., Azadirachta indica A. Juss and Moringa stenopetala Bac) against a panel of seven biofilm-forming MRSA. The leaves of the plants were extracted in organic solvents of varying polarity and the resultant crude extracts of respective medicinal plants were inspected for their antimicrobial activity by well diffusion technique. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of the plant extracts against MRSA were determined by the broth dilution method. Besides, an anti-biofilm assay of the most potent plant extract was also performed, after which its chemical constituents were delineated by combined Gas Chromatographic and Mass Spectroscopic profiling (GC-MS). The results revealed that, of the five plants, three species including M. stenopetala, R. officinalis, and O. lamifolium exhibited significant antibacterial activity. Organic solvents with high and medium polarity were excellent in extracting antimicrobials compared to nonpolar solvents. The broadest and highest rank of activity was observed in the crude ethanolic extract of M. stenopetala. Based on the MIC/MBC ratio, the crude ethanolic extract of M. stenopetala was determined to be bacteriostatic. Anti-biofilm assay showed that the extract of M. stenopetala fairly inhibited the growth of MRSA in the preformed biofilm matrix. The GC-MS analysis of M. stenopetala revealed the presence of twelve compounds with antimicrobial activity. The present study provides new insight into the development of novel drug leads to the management of MRSA.

show abstract

Section: Primary Screeningmentioning

confidence: 99%

In vitro antibacterial activity of medicinal plants against biofilm-forming methicillin-resistant Staphylococcus aureus: efficacy of Moringa stenopetala and Rosmarinus officinalis extracts

Manilal

Sabu

Shewangizaw

et al. 2020

Heliyon

View full text Add to dashboard Cite

show abstract

“…The seeds show a flocculating property, important in purifying turbid water (Abuye et al, 2003;Prashith et al, 2016). It is a major source of oil which could be important for cooking, salad (Raghavendra et al, 2016), and for different industrial applications (Seifu, 2015).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial properties of Moringa Stenopetala seed oil

Duguma¹,

Azene²,

Chameno³

et al. 2022

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Moringa stenopetala is a multipurpose tree with considerable economic and social potential as it has vital nutritional, industrial, and medicinal applications. The study was aimed to investigate the antimicrobial activities of M. stenopetala seed oil against pathogenic microorganisms. M. Stenopetala seeds were collected from three locations (Damba Gofa, Shelle, and Konso) and extracted using two different solvents (hexane and petroleum ether). Pathogenic microorganisms: bacteria (gram-positive, Staphylococcus aureus, and gram-negative Escherichia coli) and the fungal strains (Trichophyton mentagrophytes and Candida albicans) were used in this study. Standard procedures were followed to determine antimicrobial activities of M. stenopetala extract against pathogenic microorganisms. The result revealed that M. stenopetala seed extract has shown inhibitory activity against T. mentagrophytes fungi at the concentration ≥ 12.5% at all locations and both extraction solvents used. However, the extract did not show any inhibitory activity against tested bacteria and C. albicans fungi. The finding indicated that M. stenopetala seed could be used as an alternative to chemical fungicide to control T. mentagrophytes fungi. Further investigation is needed on the identification of compounds that inhibits the pathogenic microorganism.

show abstract

“…Where control and test denotes the colony diameter of test fungi on control and poisoned plates respectively [25].…”

Section: Antifungal Activity Of Leaf and Fruit Extractsmentioning

confidence: 99%

“…Respective wells were filled with 100 µl of leaf and fruit extracts (20 mg/ml of DMSO), standard antibiotic (Chloramphenicol; 1 mg/ml of sterile distilled water) and DMSO. The plates were incubated in upright position for 24 h at 37 °C and zones of inhibition formed around wells were measured [25].…”

Section: Antibacterial Activity Of Leaf and Fruit Extractsmentioning

confidence: 99%

Antimicrobial, Antiradical and Insecticidal Activity of Gardenia Gummifera L. F. (Rubiaceae)

Kekuda¹,

Raghavendra

Shilpa³

et al. 2017

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: The present study was carried out to investigate antimicrobial, antiradical and insecticidal potential of leaf and fruit of Gardenia gummifera L. f. (Rubiaceae). Methods:The leaf and fruits were shade dried, powdered and extracted by maceration process using methanol. Antibacterial activity was evaluated against Gram positive and Gram negative bacteria by Agar well diffusion assay. Antifungal activity was determined against six seed-borne fungi by Poisoned food technique. Antiradical activity of leaf and fruit extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) radical scavenging assays. Insecticidal activity of leaf and fruit extracts, in terms of larvicidal and pupicidal activity, was assessed against larvae and pupae of Aedes aegypti. Results:Both the extracts inhibited all test bacteria. Marked antibacterial activity was displayed by fruit extract when compared to leaf extract. S. epidermidis and E. coli were inhibited to highest and least extent by both extracts respectively. Fruit extract was found to exhibit higher antifungal effect when compared to leaf extract. Leaf extract and fruit extract exhibited highest inhibitory activity against A. niger and A. flavus respectively. Leaf and fruit extracts scavenged DPPH radical's dose dependently with an IC50 value of 49.01µg/ml and 2.53µg/ml respectively. The scavenging of ABTS by leaf and fruit extracts was dose dependent and the IC50 Conclusion: Overall, fruit extract of G. gummifera exhibited marked antimicrobial, antiradical and insecticidal activity when compared to leaf extract. The plant can be used for developing agents/formulations effective against infectious microorganisms, oxidative stress and insect vectors that transmit dreadful diseases. The observed bioactivities could be ascribed to the presence of active principles which are to be isolated and characterized.value for leaf and fruit extract was 2.58µg/ml and 2.31µg/ml respectively. Fruit extract was shown to exhibit marked antiradical activity when compared to leaf extract. Leaf and fruit extracts exhibited dose dependent insecticidal activity in terms of larvicidal and pupicidal activity and the susceptibility of larvae and pupae to extracts was in the order II instar larvae>IV instar larvae>pupae. Fruit extract displayed marked insecticidal potential when compared to leaf extract.

show abstract

Nutritive Composition and Antimicrobial Activity of Moringa stenopetala (Baker f.) Cufod

Cited by 10 publications

References 0 publications

In vitro antibacterial activity of medicinal plants against biofilm-forming methicillin-resistant Staphylococcus aureus: efficacy of Moringa stenopetala and Rosmarinus officinalis extracts

In vitro antibacterial activity of medicinal plants against biofilm-forming methicillin-resistant Staphylococcus aureus: efficacy of Moringa stenopetala and Rosmarinus officinalis extracts

Antimicrobial properties of Moringa Stenopetala seed oil

Antimicrobial, Antiradical and Insecticidal Activity of Gardenia Gummifera L. F. (Rubiaceae)

Contact Info

Product

Resources

About