Nutritive blood flow affects microdialysis O/I ratio for [14C]ethanol and3H2O in perfused rat hindlimb

Newman, John; Maria, Carla A.D.; Rattigan, Stephen; Clark, Michael G.

doi:10.1152/ajpheart.2001.281.6.h2731

Cited by 27 publications

(44 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extraction of [ 14 C]ethanol and 3 H 2 O from microdialysis probe perfusate has been used recently to measure total blood flow in rat muscle and to assess its distribution between nutritive and non-nutritive microvascular beds (Newman et al, 2001). However, to our knowledge, this is the first time that microdialysis probes have been used in humans in vivo to both deliver and recover solute from the cutaneous tissue space in an investigation of the impact of blood flow on the distribution of solute within the interstitial space.…”

Section: Blood Flow and Microdialysis Probe Recovery 685mentioning

confidence: 99%

“…It was used initially for the recovery of neurotransmitters from the brains of experimental animals (Bito et al, 1966). Since then it has been adapted for use in many other tissues including skin (Petersen et al, 1994;Andersson et al, 1995;Krogstad et al, 1996;Kellogg et al, 1998;Clough, 1999), adipose tissue (Lindberger et al, 2001), muscle (Tegeder et al, 1999;Newman et al, 2001) and the gastrointestinal tract (Iversen et al, 1997). It has been used to assess the transcutaneous delivery of xenobiotics and their pharmacokinetic disposition, and to explore the efficacy of percutaneous delivery devices such as iontophoresis (Stagni et al, 1999).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effects of Blood Flow on the in Vivo Recovery of a Small Diffusible Molecule by Microdialysis in Human Skin

Clough¹,

Boutsiouki²,

Church³

et al. 2002

J Pharmacol Exp Ther

View full text Add to dashboard Cite

The aim of this study was to investigate the impact of changes in local blood flow on the recovery of a small, diffusible molecule (sodium fluorescein) from the extravascular tissue space of the skin, by microdialysis in vivo. Loss and recovery of fluorescein by linear microdialysis probes (5-kDa molecular mass cutoff, 0.2 mm diameter) inserted 1 mm apart in pairs, at three sites in the skin of the volar surface of the forearm of healthy volunteers, was measured under conditions of basal, reduced (noradrenaline, 0.005 mg/ml), and increased (glyceryl trinitrate, patch) blood flow. Whereas loss of tracer from the delivery probe appeared unaffected by changes in local blood flow, retrieval of fluorescein by the second probe was directly related to blood flux, measured using scanning laser Doppler imaging. Steady-state recovery at vasoconstricted sites was 4.0 Ϯ 0.7 g ⅐ ml Ϫ1 compared with 1.8 Ϯ 0.7 g ⅐ ml Ϫ1 at control sites (p Ͻ 0.001). Local vasodilatation reduced the retrieval of fluorescein by ϳ50% to give a steadystate concentration of fluorescein in the dialysate at 40 to 50 min after the start of perfusion of 0.9 Ϯ 0.3 g ⅐ ml Ϫ1 (p ϭ 0.05). These studies in the skin are consistent with microdialysis theory. They suggest that clearance of solute by the blood will have a significant impact on microdialysis probe recovery and that, in the skin, the magnitude of this clearance is directly related to blood flow.Microdialysis is a well established technique for continuous sampling of small, water-soluble molecules within the extracellular fluid space in vivo. It was used initially for the recovery of neurotransmitters from the brains of experimental animals (Bito et al., 1966). Since then it has been adapted for use in many other tissues including skin (Petersen et al

show abstract

Section: Blood Flow and Microdialysis Probe Recovery 685mentioning

confidence: 99%

mentioning

confidence: 99%

Effects of Blood Flow on the in Vivo Recovery of a Small Diffusible Molecule by Microdialysis in Human Skin

Clough¹,

Boutsiouki²,

Church³

et al. 2002

J Pharmacol Exp Ther

View full text Add to dashboard Cite

show abstract

“…The microdialysis-ethanol technique reflects the nutritive blood flow in the local environment of the microdialysis probe, rather than gross blood flow through the limb or muscle bed. This technique has been validated against 133 Xe clearance as a technique to measure small changes in nutritive blood flow in skeletal muscle by several groups (22,48,60).…”

Section: Subjectsmentioning

confidence: 99%

Sex differences with aging in nutritive skeletal muscle blood flow: impact of exercise training, nitric oxide, and α-adrenergic-mediated mechanisms

Favor

Kraus

Carrithers

et al. 2014

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…supine or standing patient) and the integrity of the semi-permeable membrane due to blood clotting or local inflammatory reactions may influence the final results. Rather, the results are to be viewed as relative changes over time [36][37][38].…”

Section: Microdialysismentioning

confidence: 99%

Ischemic preconditioning prior to intermittent Pringle maneuver in liver resections

Winbladh

Björnsson

Trulsson

et al. 2011

J Hepato Biliary Pancreat

View full text Add to dashboard Cite

Background/Purpose Continuous inflow vascular occlusion during liver resections causes less severe ischemia and reperfusion injury (IRI) if it is preceded by ischemic preconditioning (IP) or if intermittent inflow occlusion is used during the resection. No previous clinical trial has studied the effects of adding IP to intermittent inflow occlusion. Methods Consecutive patients (n = 32) with suspicion of malignant liver disease had liver resections (minimum 2 segments) performed with inflow occlusion (intermittent clamping in a manner of 15 min of ischemia and 5 min of reperfusion repetitively; 15/5). Half of the patients were randomized to receive IP (10 min of ischemia and 10 min of reperfusion before parenchymal transection; 10/10). The patients were stratified according to volume of resection and none had chronic liver disease. The patients were followed for 5 days with microdialysis (μD). Results All patients completed the study and there were no deaths. No differences were seen between the groups regarding demographics or perioperative parameters (bleeding, duration of ischemia, resection volume, complications, and serum laboratory tests). There were no differences in alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, or prothrombin time (PT)‐INR levels, but μD revealed lower levels of lactate, pyruvate, and glucose in the IP group having major liver resections (analysis of variance; ANOVA). Nitrite and nitrate levels in μD decreased postoperatively, but no differences were seen between the groups. In one patient an elevated μD–glycerol curve was seen before the diagnosis of a stroke was made. Conclusions IP before intermittent vascular occlusion does not reduce the serum parameters used to assess IRI. IP seems to improve aerobic glucose metabolism, as the levels of glucose, pyruvate, and lactate locally in the liver were reduced, compared to controls, in patients having >3 segments resected. μD may be used to monitor metabolism locally.

show abstract

Nutritive blood flow affects microdialysis O/I ratio for [¹⁴C]ethanol and³H₂O in perfused rat hindlimb

Cited by 27 publications

References 36 publications

Effects of Blood Flow on the in Vivo Recovery of a Small Diffusible Molecule by Microdialysis in Human Skin

Effects of Blood Flow on the in Vivo Recovery of a Small Diffusible Molecule by Microdialysis in Human Skin

Sex differences with aging in nutritive skeletal muscle blood flow: impact of exercise training, nitric oxide, and α-adrenergic-mediated mechanisms

Ischemic preconditioning prior to intermittent Pringle maneuver in liver resections

Contact Info

Product

Resources

About