Nutritional requirements and growth characteristics of a biosurfactant-producingRhodococcus bacterium

Aburuwaida, AS; Banat, Ibrahim M.; Haditirto, S; Khamis, Aidee Kamal

doi:10.1007/bf02310920

Cited by 45 publications

(26 citation statements)

References 16 publications

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“…Naitali et al (1989) reported a reduction in interfacial tension of hexadecane from 26.1 to 23 mN m -1 by Pseudomonas fluorescens and 10 mN m -1 by Alcaligenes faecalis respectively. Biosurfactant produced by Rhodococcus sp., is known to decrease both, surface and interfacial tension to 27 and 1.8 mN m -1 , respectively (Abu-Ruwaida et al 1991).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Biosurfactant/Bioemulsifier Production by a Marine Bacterium

Kumar

Mody

Jha

2007

Bull Environ Contam Toxicol

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Planococcus maitriensis Anita I (NCBI GenBank Accession number EF467308) was tested for its biosurfactant/bioemulsifying efficacy. The crude extracellular polymeric substance (EPS) produced by this bacterium contained carbohydrate (12.06%), protein (24.44%), uronic acid (11%) and sulfate (3.03%). The oil spreading potential of this EPS was comparable to Triton X100 and Tween 80. This exopolymer emulsified xylene more efficiently as compared to few standard gums. It also formed stable emulsions (E (1,080)=100) with jatropha, paraffin and silicone oils. The cell free supernatant of this bacterium successfully reduced the surface tension (from 72 to 46.07 mN m(-1)). It also decreased interfacial tension of hexane and xylene. Based on the emulsifying and tensiometric properties, this bacterium or its exopolymer could be used for bioremediation, enhanced oil recovery and in cosmetics.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Biosurfactant/Bioemulsifier Production by a Marine Bacterium

Kumar

Mody

Jha

2007

Bull Environ Contam Toxicol

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“…[18]. The nutritional requirements and growth characteristics of a biosurfactant-producing Rhodococcus bacterium isolated from Kuwaiti soil have been determined [39]. While, Espuny et al [40] reported a growth-dependent production of biosurfactant by Rhodococcus sp.…”

Section: Optimisation Of Productionmentioning

confidence: 98%

Production and applications of trehalose lipid biosurfactants

Franzetti

Gandolfi

Bestetti

et al. 2010

Euro J Lipid Sci & Tech

234

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Different types of trehalose containing glycolipids are known to be produced by several microorganisms belonging to the mycolates group, such as Mycobacterium, Rhodococcus, Arthrobacter, Nocardia and Gordonia. Different structures have been elucidated particularly in Rhodococcus genus. Trehalolipids have gained increased interest for their potential applications in a number of fields due to their ability to lower interfacial tension and increase pseudosolubility of hydrophobic compounds. The most widespread application is in bioremediation technologies as such compounds are known to enhance bioavailability of hydrocarbons. In comparison to other microbial glycolipids, trehalolipids have generally showed contrasting results and achievements with both cases of inhibition and enhancement of biodegradation rates. One of the important challenges regarding potential use of trehalose lipids in a variety of applications is the optimisation of their production and downstream processing. In fact, the purification of the target biological compounds by downstream processing can account for over half the production cost in many biotechnology applications. This is especially true in the case of the Rhodococcal glycolipids, which are often bound to cellular envelopes and are usually produced along with other surface active lipids. In this review, we highlight the current knowledge of trehalolipid biosurfactant applications and the latest successful strategies employed to reduce the cost of their production.

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“…The lyophilized material was designated the acid precipitate and analyzed by thin-layer chromatography [1]. All organic solvents used were commercially distilled and of the highest available purity (Sigma-Aldrich).…”

Section: Surfactant Isolationmentioning

confidence: 99%

“…According to Bodour and Maier [9], the criterion used for selecting biosurfactantproducers is the ability to reduce the surface tension below 40 mNm - 1 . Also, Willumsen and Karlson [56], a criterion cited for emulsion-stabilizing capacity is the ability to maintain at least 50% of the original emulsion volume 24h after formation.…”

Section: Characterization Of Isolatesmentioning

confidence: 99%

“…The acidified liquid was kept at 4°C overnight, and the precipitate that formed was collected by centrifugation (17,300 g, 30 min, 4°C). The precipitate was dissolved in distilled water, the pH was adjusted to 7.0 with 1 N NaOH, and the solution was lyophilized.The lyophilized material was designated the acid precipitate and analyzed by thin-layer chromatography [1]. All organic solvents used were commercially distilled and of the highest available purity (Sigma-Aldrich).…”

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confidence: 99%

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Isolation and characterization of halophilic Archaea able to produce biosurfactants

Kebbouche-Gana

Gana

Khemili

et al. 2009

J Ind Microbiol Biotechnol

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Halotolerants microorganisms able to live in saline environments, offer a multitude of actual or potential applications in various fields of biotechnology. This is why some strains of Halobacteria from an Algerian culture collection were screened for biosurfactant production in a standard medium using the qualitative drop-collapse test and emulsification activity assay. Five of the Halobacteria strains reduced the growth medium surface tension below 40mNm-1 and two of them exhibited high emulsion-stabilising capacity. Diesel oil-in-water emulsions were stabilized over a broad range of conditions, from pH 2 to 11, with up to 35% sodium chloride or up to 25% ethanol in the aqueous phase. Emulsions were stable to three cycles of freezing and thawing. The components of the biosurfactant were determined; it contains sugar, protein and lipid. The two Halobacteria strains with enhanced biosurfactants producers designed strain A21 and strain D21 were selected to identify by phenotypic, biochemical characteristics and by partial 16S rRNA gene sequencing. The strains have Mg2+and salt growth requirements are always above 15% (w/v) salts with an optimal concentration of 15% to 20%. Analyses of partial 16S rRNA gene sequences of the two strains suggested that they were halophiles belonging to genera of the family Halobacteriaceae, Halovivax (strain A21) and Haloarcula (strain D21). To our knowledge, this a first report of biosurfactant production at such a high salt concentration . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 1Corresponding author (e-mail: kebbouchesalima@yahoo.fr). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 Linking powered by eXtyles *Manuscript Click here to download Manuscript: cleaned_up_manuscrit.doc Click here to view linked References AbstractHalotolerants microorganisms able to live in saline environments, offer a multitude of actual or potential applications in various fields of biotechnology. This is why some strains of Halobacteria from an Algerian culture collection were screened for biosurfactant production in a standard medium using the qualitative drop-collapse test and emulsification activity assay. Five of the Halobacteria strains reduced the growth medium surface tension below 40mNm -1 and two of them exhibited high emulsion-stabilising capacity. Diesel oil-in-water emulsions were stabilized over a broad range of conditions, from pH 2 to 11, with up to 35% sodium chloride or up to 25% ethanol in the aqueous phase. Emulsions were stable to three cycles of freezing and thawing. The components of the biosurfactant were determined; it contains sugar, protein and lipid. The two H...

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Nutritional requirements and growth characteristics of a biosurfactant-producingRhodococcus bacterium

Cited by 45 publications

References 16 publications

Evaluation of Biosurfactant/Bioemulsifier Production by a Marine Bacterium

Evaluation of Biosurfactant/Bioemulsifier Production by a Marine Bacterium

Production and applications of trehalose lipid biosurfactants

Isolation and characterization of halophilic Archaea able to produce biosurfactants

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