Nutritional Profile of Food Yeast Kluyveromyces fragilis Biomass Grown on Whey

Abstract

Biomass of food yeast Kluyveromyces fragilis (MTCC 188) grown on deproteinized whey supplemented with 0.8% diammonium hydrogen phosphate and 10 ppm indole-3-acetic acid, had a crude protein content of 37%. The true protein content based on nitrogen fractionation procedure was 28.1%. Total nucleic acid content was 4.82%. This amount does not appear to be toxicologically offensive. Crude fiber, ash, and lipid content of K.fragilis dry cells were found to be 4.9%, 16%, and 7.8%, respectively. Essential fatty acids of both omega-3 and omega-6 series were found present in the fat of the yeast and represented 21.5% of the total fatty acids. All the essential amino acids were present in the proteins of K. fragilis; however, sulfur containing amino acids were found in lower amounts. Calculated protein scores indicate moderate biological value. B vitamins in the biomass were present as expected, but folic acid and pyridoxine were present in high concentration.

Production of single cell protein through fermentation of a perennial grass grown on saline lands with Cellulomonas biazotea

“…Samples in triplicate were taken at different time intervals (h) to assay concentration of cell, solid substrate, crude protein, true protein, crude fibre, nitrogen free extract and RNA (Pacheco et al 1997;Paul et al 2002). Culture samples (100 ml) were centrifuged (7000 · g at 10°C for 10 min) to remove substrate.…”

“…The remaining 100 ml sample, containing cell mass and unutilized substrate was also dried (called dry biomass). It was routinely analysed for crude protein, true protein, and RNA as described previously (Pacheco et al 1997;Paul et al 2002). The micro-Kjeldahl nitrogen of dry biomass was multiplied by 6.25 to calculate crude protein.…”

“…Soluble proteins in fermentation broth were determined by the Lowry method. Total RNA content was determined using orcinol-HCl reagent as described previously (Pacheco et al 1997;Paul et al 2002) and its content was reduced by heat-treatment as mentioned earlier (Abouzeid et al 1995).…”

“…Microorganisms have the ability to upgrade low protein plant material to high protein feed. Large-scale fermentation of methanol, starch and molasses-based media have proved economically viable for the production of animal feed and human food (Rosenberg 1993;Hongpattarakere & H-Kittikun 1995;Paul et al 2002).…”

Pollutant removal-oriented yeast biomass production from high-organic-strength industrial wastewater: A review

“…Therefore, the concept of using yeasts to bioconvert highcarbohydrate wastewaters has long attracted the attention of SCP researchers. High-carbohydrate wastewater for SCP processes is produced widely in many food processing industries [13], e.g., starch processing wastewater [24], waste cassava starch hydrolysate [9], deproteinized whey [25], and defatted rice polishings [5]. Recent studies have examined different vegetable processing wastewaters for yeast biomass production (Table 1), e.g., water extracts of cabbage and watermelon [26,27], pineapple cannery effluent [8,28], silage effluent [10], sugar cane bagasse hydrolysate [14], waste capsicum powder [15], and bamboo wastewater [29].…”