Nutritional Metabolomics: What are the perspectives?

Sébédio, Jean‐Louis; Martin, Jean-François; Pujos, Estelle

doi:10.1051/ocl.2008.0230

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…The reason for this is the huge chemical diversity that is present in biological samples (79) . To progress this field the metabolomics community are actively developing databases; examples include the Human Metabolome Database, Metlin and MassBank, which are publicly available.…”

Section: Challenges and Future Outlooksmentioning

confidence: 99%

“…Another challenge is in the area of metabolite identification, which is one of the main limiting factors, particularly when using MS-based metabolomics. The reason for this is the huge chemical diversity that is present in biological samples ( 79 ) . To progress this field the metabolomics community are actively developing databases; examples include the Human Metabolome Database, Metlin and MassBank, which are publicly available.…”

Section: Challenges and Future Outlooksmentioning

confidence: 99%

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The role of metabolomics in determination of new dietary biomarkers

O’Gorman

Brennan

2017

Proc. Nutr. Soc.

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Traditional methods for the assessment of dietary intake are prone to error; in order to improve and enhance these methods increasing interest in the identification of dietary biomarkers has materialised. Metabolomics has emerged as a key tool in the area of dietary biomarker discovery and to date the use of metabolomics has identified a number of putative biomarkers. Applications to identify novel biomarkers of intake have in general taken three approaches: (1) specific acute intervention studies to identify specific biomarkers of intake; (2) searching for biomarkers in cohort studies by correlating to self-reported intake of a specific food/food group(s); (3) analysing dietary patterns in conjunction with metabolomic profiles to identify biomarkers and nutritypes. A number of analytical technologies are employed in metabolomics as currently there is no single technique capable of measuring the entire metabolome. These approaches each have their own advantages and disadvantages. The present review will provide an overview of current technologies and applications of metabolomics in the determination of new dietary biomarkers. In addition, it will address some of the current challenges in the field and future outlooks.

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Section: Challenges and Future Outlooksmentioning

confidence: 99%

Section: Challenges and Future Outlooksmentioning

confidence: 99%

The role of metabolomics in determination of new dietary biomarkers

O’Gorman

Brennan

2017

Proc. Nutr. Soc.

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“…11,12 Recently, due to being a powerful and versatile tool, NMR based metabolomics have been highlighted in nutrition research. 9,10,13 The NMR based global metabolomics methods involved in food nutrition research usually produce high-dimensional and complex data sets. Multivariate pattern recognition techniques including principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), or orthogonal partial least squares discriminant analysis (OPLS-DA) are oen used to reduce the dimensionality of the data.…”

Section: Introductionmentioning

confidence: 99%

¹H-NMR metabolomics analysis of nutritional components from two kinds of freshwater fish brain extracts

Wang

et al. 2018

RSC Adv.

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“…As part of our ongoing study (METAPROFILE) on the identification of early biomarkers of weight gain in humans (Sébedio et al 2008), we have developed and validated a UPLC-MS method to analyze human plasma. Plasma was selected as it contains erythrocytes whose lipid composition is known to reflect dietary patterns (Gibney and Gibney 2004;Skeaff et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a UPLC/MS method for a nutritional metabolomic study of human plasma

et al. 2009

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In order to study the effect of a diet on metabolites found in body fluids such as plasma, we have developed and validated a UPLC/MS method. While methods using NMR have been well established to analyse different biological tissues, recent studies have described robust untargeted UPLC-MS methods for plasma analysis. One major concern when profiling plasma is the presence of an important quantity of proteins which have to be precipitated without any loss of metabolites prior to LC/MS analysis. The utilization of untargeted approaches in nutritional metabolomics still suffers from the lack of identification of specific biomarkers. We therefore suggest an alternative method still using a global approach but focusing at the same time on metabolites previously described in human plasma in order to detect biomarkers of metabolic dysregulations. Thus, to fulfil our objectives, analytical parameters were tested (i) the anticoagulant type for sample collection, (ii) the protein precipitation method and (iii) UPLC/MS analytical conditions. Three protein precipitation methods and two anticoagulants were tested and compared. The method utilizing blood collection on heparin and methanol precipitation was chosen for giving the most reproducible results while keeping the complexity of the sample. Finally, a validation was proposed to evaluate the stability of this analytical method applied to a large batch of samples for nutritional metabolomic studies.

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Nutritional Metabolomics: What are the perspectives?

Cited by 3 publications

References 31 publications

The role of metabolomics in determination of new dietary biomarkers

The role of metabolomics in determination of new dietary biomarkers

¹H-NMR metabolomics analysis of nutritional components from two kinds of freshwater fish brain extracts

Development and validation of a UPLC/MS method for a nutritional metabolomic study of human plasma

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Nutritional Metabolomics: What are the perspectives?

Cited by 3 publications

References 31 publications

The role of metabolomics in determination of new dietary biomarkers

The role of metabolomics in determination of new dietary biomarkers

1H-NMR metabolomics analysis of nutritional components from two kinds of freshwater fish brain extracts

Development and validation of a UPLC/MS method for a nutritional metabolomic study of human plasma

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¹H-NMR metabolomics analysis of nutritional components from two kinds of freshwater fish brain extracts