Nutritional Enhancement of Chicken Feather Waste by Bacillus aerius NSMk2

Bhari, Ranjeeta; Kaur, Manpreet; Singh, Ram Sarup

doi:10.1007/s12088-020-00897-0

Cited by 18 publications

(4 citation statements)

References 22 publications

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“…However, the type and amount of the released AA differ according to the substrate, microbe, and condition of fermentation. For instance, the biodegradation of chicken feather waste by B. aerius NSMk2 released 17 free AA, where eight were essential (Bhari et al, 2020). B. cereus PCM 2849 was able to release both essential and non-essential AA where the glutamic acid and proline were found at the highest concentrations (Ciurko et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Innovative Artificial-Intelligence- Based Approach for the Biodegradation of Feather Keratin by Bacillus paramycoides, and Cytotoxicity of the Resulting Amino Acids

et al. 2021

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The current study reported a new keratinolytic bacterium, which was characterized as Bacillus paramycoides and identified by 16S rRNA, and the sequence was then deposited in the GenBank (MW876249). The bacterium was able to degrade the insoluble chicken feather keratin (CFK) into amino acids (AA) through the keratinase system. The statistical optimization of the biodegradation process into AA was performed based on the Plackett–Burman design and rotatable central composite design (RCCD) on a simple solid-state fermentation medium. The optimum conditions were temperature, 37°C, 0.547 mg KH2PO4, 1.438 mg NH4Cl, and 11.61 days of incubation. Innovatively, the degradation of the CFK process was modeled using the artificial neural network (ANN), which was better than RCCD in modeling the biodegradation process. Differentiation of the AA by high-performance liquid chromatography (HPLC) revealed the presence of 14 AA including essential and non-essential ones; proline and aspartic acids were the most dominant. The toxicity test of AA on the HepG2 cell line did not show any negative effect either on the cell line or on the morphological alteration. B. paramycoides ZW-5 is a new eco-friendly tool for CFK degradation that could be optimized by ANN. However, additional nutritional trials are encouraged on animal models.

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Section: Discussionmentioning

confidence: 99%

Innovative Artificial-Intelligence- Based Approach for the Biodegradation of Feather Keratin by Bacillus paramycoides, and Cytotoxicity of the Resulting Amino Acids

et al. 2021

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“…Keratinases are extracellular inducible proteases that hydrolyze crystalline and cross-linked keratin structures to produce a keratin hydrolysate which consists mainly of soluble proteins, peptides and amino acids [16]. Keratinase production can be significantly enhanced by modifying production medium and process parameters.…”

Section: Discussionmentioning

confidence: 99%

“…During the fermentation period, rheological properties of broth may change significantly due to increase in biomass and changes in morphological structure of keratin substrate [32]. Keratinase has been reported to degrade keratinous biomass faster with the simultaneous release of proteins, peptides and amino acids [16]. These secondary metabolites increase viscosity of liquid medium that subsequently influences mass transfer and reduces enzyme production [8].…”

Section: Discussionmentioning

confidence: 99%

Optimization and validation of keratinase production by Bacillus aerius NSMk2 in a stirred tank reactor using response surface methodology

2021

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Keratinase is a robust enzyme that is produced in the presence of keratin substrates. This enzyme has been recognized for its applications in waste management, leather and detergent industries. Our group has isolated a potential keratinase producing strain of Bacillus aerius NSMk2 from poultry dump soil, and its hide dehairing and stain removal applications have been studied. Considering commercial applicability of keratinase, the present study reports the keratinase production in a stirred tank reactor (5 l). Central composite rotatable design of response surface methodology (RSM) was employed to study the effect of most influencing process variables, i.e., aeration (0.5–1.5 vvm), agitation (150–350 rpm) and incubation period (24–48 h) on keratinase production. The quadratic model predicted 15 experimental runs, and the influence of independent variables and their interaction on keratinase production were interpreted using analysis of variance (ANOVA) and t-test statistics. Coefficient of determination (R2) value close to 1 and Fisher F-value of 3743.77 showed good fit of experimental data to second-order polynomial equation. A reasonable agreement between experimental and predicted values showed the accuracy of deduced model. Applying the desirability function, aeration rate of 1.0 vvm, agitation rate of 276.88 rpm and incubation period of 33.68 h supported maximum keratinase production (318.38 U/ml). Confirmatory experiments were performed to evaluate the accuracy of desirability function. Maximum keratinase activity of 318.11 U/ml close to predicted value (318.38 U/ml) validates the model. The present study provides useful guidelines for large-scale production of keratinase that can be used for various commercial applications. Article highlights Keratinase production was optimized in a stirred tank reactor by RSM. The influence of aeration, agitation and incubation period on keratinase production was studied. A significant increase in keratinase production was observed at 1.0 vvm aeration and 276.88 rpm agitation after 33.68 h.

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“…Максимальный выход белков и аминокислот регистрировали при рН 7,5, 37 C и встряхивании при 175 об/мин. Полный гидролиз белых куриных перьев произошел через 84 ч (29).…”

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NEW IDENTIFIED Bacillus licheniformis ISOLATES ENSURE ENZY-MATIC BIODEGRADATION OF FEATHERS INTO A FEED ADDITIVE

Betekhtin

2024

S-h. biol.

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Биодеградация кератина пера сельскохозяйственной птицы микроорганизмами, обладающими кератинолитической активностью, представляет альтернативный привлекательный метод повышения питательной ценности отходов и предотвращения загрязнения окружающей среды. Кератинолитические протеазы используются для получения редких аминокислот -пролина, серина и цистеина. Бактериальный гидролизат перьев можно применять в качестве ингредиента корма для животных или органического удобрения, благодаря чему снижается воздействие отходов птицеводства на окружающую среду. В настоящей работе впервые установлено, что при переработке пера цыплят-бройлеров штаммом Bacillus licheniformis БАЛ-2, который использует перо в качестве единственного источника углерода, свободный кератин полностью превращается в перевариваемые пептиды и незаменимые аминокислоты. Цель работы заключалась в изучении морфологических, физиолого-биохимических и молекулярно-генетических свойств штаммов Bacillus licheniformis БАЛ-1 и БАЛ-2, их идентификации и использования для ферментации пера цыплят-бройлеров. Штаммы Bacillus licheniformis БАЛ-1 (ВКПМ В-14243) и БАЛ-2 (ВКПМ В-14244) -природные изоляты, выделенные из донных отложений Финского залива Балтийского моря (д. Кандикюля). Куриное перо цыплят-бройлеров кросса Ross 308 (Птицеперерабатывающий комплекс «Константиново», ПАО «Группа Черкизово», с. Константиново, Московская обл., Раменский р-н) поступило с промышленной линии ощипывания птицы, имело включения крови, влажность 75 % и подвергалось ферментации через 24 ч после получения. Морфотипы колоний B. licheniformis определяли на триптон-соевом агаре. Геномную ДНК бактерий выделяли с использованием набора реагентов («Thermo Scientific», Литва). Фрагмент 16S рДНК амплифицировали в ПЦР со специфичными праймерами для гена 16S рРНК эубактерий: 8f 5-AGAGTTTGATCMTGGCTCAG-3 и 1492r 5-TACGGHTACCTTGTTACGACTT-3. Идентичность нуклеотидных последовательностей анализировали с помощью программы BLASTN (http://www.ncbi.nlm.nih.gov/BLAST). Для ферментации пера в колбу, содержащую 100 см 3 минерально-питательной среды, помещали 5 г измельченного пера птицы. Штамм B. licheniformis БАЛ-1 или БАЛ-2 вносили в минеральную среду до конечной концентрации 1½10 6 КОЕ/см 3 . Ферментацию пера проводили в течение 72 ч при 28 С и постоянном перемешивании. В культуральной жидкости определяли кератиназную активность с использованием суспензии кератина. Раствор фермента (1 см 3 ) добавляли к суспензии кератина (1 см 3 ) в реакционном буфере. После 1 ч инкубации при 37 C реакцию останавливали добавлением 2 см 3 5 % ТХУ и раствор фильтровали. Ферментированное перо исследовали методом растровой электронной микроскопии. Двумерный гель-электрофорез выполняли по О'Фарреллу c изоэлектрофокусированием в амфолиновом градиенте pH (IEF-PAGE); белки детектировали последовательным окрашиванием кумасси голубым R-250 и азотнокислым серебром. При подготовке препаратов для 2D электрофореза 100 мг измельченного образца гомогенизировали в 2 см 3 лизирующего раствора. Гомогенат осветляли центрифугированием, фракцию ...

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Nutritional Enhancement of Chicken Feather Waste by Bacillus aerius NSMk2

Cited by 18 publications

References 22 publications

Innovative Artificial-Intelligence- Based Approach for the Biodegradation of Feather Keratin by Bacillus paramycoides, and Cytotoxicity of the Resulting Amino Acids

Innovative Artificial-Intelligence- Based Approach for the Biodegradation of Feather Keratin by Bacillus paramycoides, and Cytotoxicity of the Resulting Amino Acids

Optimization and validation of keratinase production by Bacillus aerius NSMk2 in a stirred tank reactor using response surface methodology

NEW IDENTIFIED Bacillus licheniformis ISOLATES ENSURE ENZY-MATIC BIODEGRADATION OF FEATHERS INTO A FEED ADDITIVE

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