Nutritional and supranutritional levels of selenate differentially suppress prostate tumor growth in adult but not young nude mice

Opposing impacts on healthspan and longevity by limiting dietary selenium in telomere dysfunctional mice

“…A total of 203 weanling G3 Terc −/− mice were fed an AIN‐93G Torula yeast Se‐deficient basal diet (Se−, 0.03 ppm by analysis) or the diet supplemented with Se at 0.15 ppm (nutritionally adequate, Se+) as sodium selenate (Holmstrom et al ., ) until they were sacrificed or died of natural causes. Based on the log‐rank test of equality of two survival functions (Peto & Peto, ), Se− G3 Terc −/− mice surprisingly exhibited a higher survival probability than Se+ mice over time in both males ( P = 0.045, Fig.…”

“…Our diets for adult mice are based on AIN‐93G instead of AIN‐93M because the former includes greater amount of fat and protein that better represents the dietary components of Western foods. The basal (Se−) diet contained 30% Torula yeast and 0.03 mg kg −1 Se by analysis (AOAC Method 986.15; Covance Laboratory, Madison, WI, USA) and was supplemented with Se at 0.15 mg kg −1 (Se+) as sodium selenate (Holmstrom et al ., ). Selenate is the form of Se in standard AIN‐93 diets and is considered to be more bioavailable and less toxic than selenite.…”

“…injected with insulin (0.25 unit kg body weight −1 ; Sigma‐Aldrich, St. Louis, MO, USA). Area under curve analyses (Stephens et al ., ) and plasma Se concentrations (Holmstrom et al ., ) were determined as previously described. Plasma Gpx3 protein was analyzed by Western analysis using 0.3 μL plasma and anti‐Gpx3 (1:2000) and anti‐albumin (1:2000) antibodies (R&D Systems, Minneapolis, MN, USA).…”

In VivoandIn VitroAntitumor Effects of Platycodin D, a Saponin Purified from Platycodi Radix on the H520 Lung Cancer Cell

“…Nude mice are congenitally athymic with T lymphocyte related immune deficiency and have served as a useful model for assessing tumor xenograft development [ 44 , 45 ]. Interestingly, nude mice develop an age-dependent extrathymic T cell maturation, the extent of which is greater in CD8+ cytotoxic than in CD4+ helper T cells [ 46 , 47 ]. Therefore, it also considered that some immunomodulatory agents can be showed antitumor effect itself or synergic effects with cytotoxic agents mediated by immune stimulated effects, because tumor is directed related with immune deficiency [ 46 , 48 ].…”

Human Lung Cancer Cell Line A-549 ATCC Is Differentially Affected by Supranutritional Organic and Inorganic Selenium

“…Even when high Se doses may be toxic [13, 14], supranutritional levels of this element exert protective effects against some diseases including hypertension and cancer [15–19]. Although the anticarcinogenic effects of Se have been demonstrated [20] to our knowledge, few studies compare the effects when different chemical forms of Se are supplied at supranutritional levels.…”