Nutrition of the Green Alga Golenkinia

Abstract

Defined media were established for four strains of Golenkinia. Strains 929 and 930 require thiamine and vitamin B12, strain 931 requires the latter and is stimulated by thiamine; only strain 320 and a mutant of strain 931 are capable of completely autotrophic growth. The optimal nitrogen concentration is 0.025 m except for strain 931 where it is 0.015 m and the requirement may be met by nitrate, ammonium, or urea. Optimal levels of the other components are: 0.0025 m KH2PO4, 0.0005 to 0.001 m MgSO4, and 50 ml of a stock trace‐element solution per liter. Only strain 931 required calcium; however, its addition stimulates the growth of strains 929 and 930. The optimal pH before autoclaving is 6.8. Maximal growth rates of two to three cell doublings per day and yields of 26 to 30 (log2) cells per ml were obtained. These growth data compare favorably with those for other unicellular green algae.

Defined Media for Growth and Gamete Production by the Green Alga, Oedogonium cardiacum

“…The maximal dry weights obtained (the final points in Fig. 1) compare favorably with those reported for other green algae (1). development (lower).…”

The effects of nutrient, pH and herbicide levels on algal growth

“…Morever, it was indicated that Ca and. Mg are widely interchangeable (20). Thus Ca levels tested in this research could be affected by the presence of Mg in the media and vice versa.…”

Factors affecting spore germination in algae — review

“…Vitamins are required to stimulate growth in many algae (Machlis 1962;Ellis and Machlis 1968;Saito 1972;Dawson and Denny 1983). Ascorbic acid (vitamin C) and serine at certain levels increased akinete GRM in Stigeoclonium pascheri (Agrawal 1988a).…”