Nutrient transport in bioreactors for bone tissue growth: Why do hollow fibre membrane bioreactors work?

Glucose diffusivity in cell-seeded tissue engineering scaffolds

“…The main goal of tissue engineering is to design, construct, regenerate and repair damaged tissues in the human body (Abdullah et al 2009;Ahn et al 2014). …”

A magnetic resonance-compatible perfusion bioreactor system for three-dimensional human mesenchymal stem cell construct development

“…optical fluorescence. While the current study demonstrated the potential of the MR-compatible perfusion bioreactor for hMSC construct development, its capability can be further enhanced by introducing cell labeling agents and molecular probes to overcome limitation in specificity and resolution (Guzman et al, 2007;Kamaly et al, 2008;Massoud and Gambhir, 2003) and by modeling analysis of shear stress and transport behaviors during seeding and cultivation (Zhu et al, 2010;Abdullah et al, 2008). Furthermore, the current study utilizes only 1 H MRS to analyze the bioreactor.…”

A strategy to determine operating parameters in tissue engineering hollow fiber bioreactors

“…There is extensive understanding of HFB fluid dynamics and mass transport obtained from experimental and modeling studies, and a wealth of data on tissue physiology and cell metabolism in vivo and in vitro. For example, Abdullah et al (2) and Abdullah and Das (1) have focused on high-density bone cell populations, whereas hepatocyte culture has provided a focus for bioartificial liver development through studies such as Hay et al (14), Kawazoe et al (16), Nyberg et al (24), Patzer (27), Sielaff et al (33), Sullivan et al (35), and Wurm et al (40). Together these studies provide insight into the interaction between the cell environment and the fluid dynamics and mass transfer of nutrients across the membrane.…”