Nutrient Requirements of Methanogenic Bacteria

Bryant, M. P.; Tzeng, Sinfu; Robinson, I. M.; Joyner, A. E.

doi:10.1021/ba-1971-0105.ch003

Cited by 149 publications

(79 citation statements)

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“…The sterile samples were inoculated with 4 g marshland soil and 20 ml medium was added. The carbonate-buered medium had the following composition: 250 ml mineral soil solution (extracted from 200 g marshland soil), 250 ml dissolved organic carbon solution (extracted from 1000 g marshland soil, about 40 mg DOC l À 1 ), 5 ml trace element solution (Wolin et al, 1963), 10 ml vitamin solution (Bryant et al, 1971) and 490 ml distilled water. The model soils were incubated at 288C for 12 months in darkness.…”

Section: Incubation Experiments With Dierent Textured Model Soilsmentioning

confidence: 99%

Methane production in aerated marshland and model soils: effects of microflora and soil texture

Wagner

Pfeiffer

Bock

1999

Soil Biology and Biochemistry

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Under oxic conditions the importance of the indigenous micro¯ora and the soil texture on methane production was investigated using marshland soils (clayey silt) and dierent textured model soils (clay, sand, gravel). Under an oxic partial pressure >2.5% O 2 , soil slurries had a low methane production rate over a period of 50±70 h with acetate (0.35 2 0.1 nmol g) as well as with hydrogen (2.432 0.51 nmol g À 1 h À 1 ) as substrate. The rates amounted to 1 and 5%, respectively, compared to methane production rates under anoxic conditions. As soon as the oxygen concentration decreased to 2.5% (microaerophilic conditions) the methane production increased signi®cantly (9.52 3.3 nmol g À 1 h À 1 with acetate; 16.3 2 3.3 nmol g À 1 h À 1 with hydrogen). In the absence of the indigenous micro¯ora, the inoculated culture of Methanosarcina mazei (DSM 2053) did not produce any methane in soil slurries under oxic conditions. To inhibit methane oxidation, all samples (oxic and anoxic) were supplied with 60 nl acetylene ml À 1 headspace. Furthermore, methane production in dierent textured model soils demonstrated that a high amount of negative surface charges increased methane production under oxic as well as under anoxic conditions. Consequently the methane production rates increased in the following sequence: sand < gravel < clayey silt (marshland soil)clay. Our results show that the indigenous micro¯ora in combination with the sorptive quality of soil particles (clay, silt, organic matter) enables methanogenic activity in the presence of oxygen, promoting microscale anoxia within the slurries. #

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Section: Incubation Experiments With Dierent Textured Model Soilsmentioning

confidence: 99%

Methane production in aerated marshland and model soils: effects of microflora and soil texture

Wagner

Pfeiffer

Bock

1999

Soil Biology and Biochemistry

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“…Methanogens, without exception, can utilize ammonia as a nitrogen source for growth (41), although recently, it has been shown that Methanobacterium ivanovi can also utilize glutamine and that Methanobacterium thermoautotrophicum delta H is capable of growth on urea or glutamine (10) as a nitrogen nutrient source. Bryant et al (12) studied the nitrogen requirements of Methanobacterium strain MOH and Methanobacterium ruminantium. These organisms required ammonia as a nitrogen source with relatively little nitrogen being incorporated from amino acids supplied.…”

mentioning

confidence: 99%

Purification and characterization of glutamine synthetase from the archaebacterium Methanobacterium ivanovi

Bhatnagar¹,

Zeikus²,

Aubert³

1986

J Bacteriol

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Glutamine synthetase (GS) was purified to electrophoretic homogeneity from the obligate anaerobic archaebacterium Methanobacterium ivanovi. The 130-fold-purified enzyme was obtained by heat treatment, ion-exchange chromatography, and gel filtration. Like all other eubacterial GSs known so far, the GS of M. ivanovi was found to be a dodecamer of about 600,000 daltons composed of a single type of subunit. The enzyme was stable at 63°C for 10 min and was not sensitive to oxygen. The isoelectric point was 4.6, and the optimum pH of gamma-glutamyltransferase activity was 8.0. The Km values for hydroxylamine, glutamine, and ADP in the transferase reaction were 6.8, 22.7, and 0.35 mM, respectively. L-Methionine-DL-sulfoximine strongly inhibited the activity. Like the GS from gram-positive bacteria, Anabaena sp., several yeasts, and mammals, the enzyme from M. ivanovi was not regulated by adenylylation as demonstrated by snake venom phosphodiesterase treatment. Inhibition of the trahisferase activity by L-alanine, glycine, L-histidine, and L-tryptophan was observed. L-Glutamine alone or in the presence of AMP did not inhibit the GS synthetic activity. The GS of Methanobacterium ivanovi did not cross-react with a variety of antisera against GS from Escherichia coli, Anabaena strain 7120, or Bacillus megaterium. Archaebacterial GS appears to be structurally and functionally similar to eubacterial GS in gram-positive bacteria.Glutamine synthetase (GS) (L-glutamate:ammonia ligase (ADP); EC 6.3.1.2) plays a key role in the ammonia assimilation in many procaryotes and eucaryotes since it catalyzes the first step in a metabolic pathway that leads finally to the synthesis of nearly all the important macromolecules of the cell (23, 28). It has been extensively studied at the biochemical and molecular level in various bacteria (2, 28, 36) and cyanobacteria (25,29,34,36

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“…The culture was grown in 15-ml glass flasks on Bryant's medium (Bryant and Robinson, 1961). It contained 0.5 per cent sodium acetate, 3 per cent NaCl, and 0.5 per cent vitamin solution (Bryant et al, 1971) instead of rumen fluid and carbohydrates. The gas phase consisted of 75 per cent H 2 and 25 per cent CO 2 .…”

Section: Methodsmentioning

confidence: 99%

A Short Report on Microbiology of Sediments from Deep Sea Drilling Project Holes 415, 415A, and 416A

Ivanov¹,

Belyaev²,

Laurinavichus³

1980

Initial Reports of the Deep Sea Drilling Project

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Nutrient Requirements of Methanogenic Bacteria

Cited by 149 publications

References 0 publications

Methane production in aerated marshland and model soils: effects of microflora and soil texture

Methane production in aerated marshland and model soils: effects of microflora and soil texture

Purification and characterization of glutamine synthetase from the archaebacterium Methanobacterium ivanovi

A Short Report on Microbiology of Sediments from Deep Sea Drilling Project Holes 415, 415A, and 416A

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