“…Near-infrared spectrophotometry (NIRS, Model: FOSS NIRSystems 5000, Laurel, MD, USA) was used to analyse organic matter (OM), water-soluble carbohydrates (WSCs), neutral and acid detergent fibre (NDF, ADF), crude protein (CP), and DM, OM, and dry OM digestibility (DMD, OMD, DOMD, respectively). The metabolizable energy (ME) content of forage was calculated as per the calculation of the primary Industries Standing Committee (2007) [33] of 0.16 × DOMD. NIRS calibration equations were determined prior to sample analysis for OM by digestibility (R 2 = 0.89) [34], WSC (R 2 = 0.97) [35], ADF (R 2 = 0.97) (AOAC, 1990 [36]; method 973.18), NDF (R 2 = 0.98) [37], CP by combustion (R 2 = 0.99) (Vario Max CN Analyser Elementar, Langenselbold, Germany), DMD (R 2 = 0.97), OMD (R 2 = 0.97), and DOMD (R 2 = 0.92) [34].…”