Nutrient remediation rates in municipal wastewater and their effect on biochemical composition of the microalga Scenedesmus sp. AMDD

Dickinson, Kathryn E.; Whitney, Crystal G.; McGinn, Patrick J.

doi:10.1016/j.algal.2013.01.009

Cited by 80 publications

(45 citation statements)

References 62 publications

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“…For wastewater remediation of nitrate and phosphate, S. quadricauda is the most suitable species showing the highest removal rates of these nutrients. These findings correlate with previous reports that have identified S. quadricauda as an effective species for nutrientrich wastewater remediation (Martıńez et al 2000;Shi et al 2007;Dickinson et al 2013).…”

Section: Discussionsupporting

confidence: 94%

“…In general, biomass production was lower than in previous reports for S. quadricauda and other chlorophytes being examined for biotechnological potential (Patil 1991;Tiftickjian and Rayburn 1986;Zhou et al 2011;Dickinson et al 2013). Lower biomass content was likely due to light limitation, as the current study was performed under controlled laboratory conditions with an average light intensity of 40 μmol photons m −2 s −1 compared to 100-200 μmol photons m −2 s −1 (Tiftickjian and Rayburn 1986;Dickinson et al 2013) or natural daylight (Patil 1991). This is of little concern, as the aim was to identify suitably halotolerant freshwater species, and culture conditions were not optimized to maximize productivity.…”

Section: Discussioncontrasting

confidence: 81%

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Salinity tolerance of four freshwater microalgal species and the effects of salinity and nutrient limitation on biochemical profiles

2015

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Microalgae are ideal candidates for bioremediation and biotechnological applications. However, salinity and nutrient resource availability vary seasonally and between cultivation sites, potentially impacting on biomass productivity. The aim of this study was to screen pollutant-tolerant freshwater microalgae (Desmodesmus armatus, Mesotaenium sp., Scenedesmus quadricauda and Tetraedron sp.), isolated from Tarong power station ash-dam water, for their tolerance to cultivation at a range of salinities. To determine if biochemical composition could be manipulated, the effects of 4-day nutrient limitation were also determined. Microalgae were cultured at 2, 8, 11 and 18 ppt salinity, and nutrient uptake was monitored daily. Growth, total lipid, fatty acid (FA), and amino acid contents were quantified in biomass harvested while nutrient-replete and, after 4 days, nutrient-deplete. D. armatus showed the highest salinity tolerance actively growing in up to 18 ppt while Mesotaenium sp. was the least halotolerant with decreasing growth rates from 11 ppt. However, Mesotaenium sp. at 2 and 8 ppt had the highest biomass productivity and nutrient requirements of the four species, making it ideal for nutrient remediation of eutrophic freshwater effluents. Salinity and nutrient status had minimal influence on total lipid and FA contents in D. armatus and Mesotaenium sp., while nutrient depletion induced an increase of total lipid and FAs in S. quadricauda and Tetraedron sp., which was further increased with increasing salinity. As none of the growth conditions affected amino acid profiles of the species, these findings provide a basis for species selection based on site-specific salinity conditions and nutrient resource availability.

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Section: Discussionsupporting

confidence: 94%

Section: Discussioncontrasting

confidence: 81%

Salinity tolerance of four freshwater microalgal species and the effects of salinity and nutrient limitation on biochemical profiles

2015

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“…A relative independence of the FAs profile regarding the different culture medium conditions is not consistent with the results for C. vulgaris cultivated in optimized BBM+DC, probably due to differences between the strains (Francisco et al 2010;Dickinson et al 2013) or culture media (Li et al 2011). At the same time, the use of optimized BBM DC as an alternative culture medium in an autotrophic system in this study enabled the production of C. vulgaris biomass characterized by a content of FAs similar to that found in the biomass of Chlorella cultivated in a soy whey culture (Mitra et al 2012).…”

Section: Discussioncontrasting

confidence: 70%

“…Furthermore, the development of culture media based on wastewater has become an important area of study, resulting in a large number of culture media obtained from different types of mineral media, organic substrates, and synthetic or real wastewaters (Orpez et al 2009;Biller et al 2012;Dickinson et al 2013;Tuantet et al 2014). …”

Section: Introductionmentioning

confidence: 99%

Optimization of biomass production of Chlorella vulgaris grown in desalination concentrate

et al. 2014

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Desalination concentrate (DC) is a potential medium for microalgal cultivation since it contains an abundance of mineral salts. In this study, the feasibility of growing Chlorella vulgaris with DC was evaluated and the influence of DC concentration on the growth, biomass productivity, and biochemical composition was assessed. Media comprised of DC mixed with bold basal medium (BBM) in different concentrations were prepared for microalga cultivation. A sequential statistical methodology, comprising fractional factorial design and response surface methodology (RSM) was applied to enhance the biomass production. The growth of C. vulgaris was promoted with 75 % BBM+25 % DC compared with the BBM control. C. vulgaris biomass production (0.59 g L −1 ) was observed with 75 % BBM+25 % DC with optimal concentrations of sodium nitrate (0.09 g L −1 ), monobasic potassium phosphate (0.014 g L −1 ), and micronutrient solution (0.20 mL L −1 ). The gradual increase of DC had a strong effect on the biochemical composition of C. vulgaris. Carbohydrate fiber and protein contents were estimated to be 14.4 and 51.3 %, respectively, when the microalgae were cultivated in 75 % BBM +25 % DC. The lipid content (12.5 %) of C. vulgaris cells was highest at the optimized BBM DC concentrations. When grown in the BBM control and optimized BBM+DC media, C. vulgaris produced lipids rich in polyunsaturated fatty acids (C18:3 ω3) and monounsaturated fatty acids (C18:1). These results indicated that DC is a feasible alternative for the mass cultivation of C. vulgaris.

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“…important for robust growth in the unsterilized wastewater containing a variety of contaminants. Previous studies have indeed reported on the potential application to wastewater and tolerance to ammonia for both C. sorokiniana (de Bashan et al 2008;Kim et al 2013a;Kobayashi et al 2013;Ogbonna et al 2000;Tuantet et al 2013Tuantet et al , 2014 and Scenedesmus species (Bjornsson et al 2013;Dickinson et al 2013;Gantar et al 1991;McGinn et al 2012;Park et al 2010), although in most of those cases, the wastewater was sterilized or filtered through 0.22-μm filter to remove bacteria and other indigenous microorganisms prior to algal cultivation. Another factor contributing to robustness of the C. sorokiniana and Scenedesmus species in wastewater may be the tough structure of their cell wall.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate

Bohutskyi

Liu

Nasr

et al. 2015

Appl Microbiol Biotechnol

111

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Eighteen microalgae, including two local isolates, were evaluated for their ability to grow and remove nutrients from unsterilized primary or secondary wastewater effluents as well as wastewater supplemented with nutrient-rich anaerobic digester centrate (ADC). Most of the tested species except several phylogenetically clustered Chlorella sorokiniana including local isolates and Scenedesmus strains were unable to grow efficiently. This may reflect the presence of certain genetic traits important for robust growth in the unsterilized wastewater. The maximum algal-specific growth rates and biomass density obtained in these bacterial-contaminated cultures were in the range of 0.8-1 day(-1) and 250-350 mg L(-1), respectively. ADC supplementation was especially helpful to biologically treated secondary effluent with its lower initial macronutrient and micronutrient content. As a result of algal growth, total nitrogen and orthophosphate levels were reduced by as much as 90 and 70 %, respectively. Biological assimilation was estimated to be the main mechanism of nitrogen removal in primary and secondary effluents with ammonia volatilization and bacterial nitrification-denitrification contributing for cultures supplemented with ADC. Assimilation by algae served as the principal mechanism of orthophosphate remediation in secondary wastewater cultures, while chemical precipitation appeared also to be important for orthophosphate removal in primary wastewater. Overall, cultivation of microalgae in primary and primary + 5 % ADC may be more favorable from an economical and sustainability perspective due to elimination of the costly and energy-intensive biological treatment step. These findings demonstrate that unsterilized wastewater and ADC can serve as critical nutrient sources for biomass generation and that robust microalgae can be potent players in wastewater phytoremediation.

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Nutrient remediation rates in municipal wastewater and their effect on biochemical composition of the microalga Scenedesmus sp. AMDD

Cited by 80 publications

References 62 publications

Salinity tolerance of four freshwater microalgal species and the effects of salinity and nutrient limitation on biochemical profiles

Salinity tolerance of four freshwater microalgal species and the effects of salinity and nutrient limitation on biochemical profiles

Optimization of biomass production of Chlorella vulgaris grown in desalination concentrate

Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate

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