“…The fresh desilked pupae were used to estimate the contents of moisture, crude protein, crude fat, crude fiber, total carbohydrate, and ash in the silkworm pupae following standard procedures [ 24 , 25 , 26 ]. The moisture content of the silkworm was determined by drying it in an oven at 105 °C, and data were obtained until a constant weight loss was achieved.…”