A mass-balance budget of N cycling was developed for an intensive agricultural area in west-central Minnesota to better understand NO 3 -contamination of ground water in the Otter Tail outwash aquifer. Fertilizer, biological fixation, atmospheric deposition, and animal feed were the N sources, and crop harvests, animal product exports, volatilization from fertilizer and manure, and denitrification were the N sinks in the model. Excess N, calculated as the difference between the sources and sinks, was assumed to leach to ground water as NO 3 -. The budget was developed using ground water data collected throughout the 212-km 2 study area. Denitrification was estimated by adjusting its value so the predicted and measured concentrations of NO 3 -in ground water agreed. Although biological fixation was the largest single N source, most was removed when crops were harvested, indicating that inorganic fertilizer was the primary source of N reaching the water table. It was estimated that denitrification removed almost half of the excess NO 3 -that leached below the root zone. Even after accounting for denitrification losses, however, it was concluded that the ground water system was receiving approximately three times as much N as would be expected under background conditions. The N cycle of agro-ecosystems is by definition dominated by agricultural sources and sinks, the magnitude of which greatly exceeds natural cycles that evolved over many millennia. Agricultural inputs of N in the USA have increased 20-fold in the past 50 yr, and the most dramatic increases have taken place in the past 30 yr (Puckett, 1995). Vitousek (1994) has estimated that of the total N used by humans throughout history up to 1992, approximately half was applied from 1982 to 1992. Consequently, many agro-ecosystems and their associated ground water and surface water systems may still be receiving increasing N amounts.One of the least appreciated components of the alteration of the natural cycle is the dramatic increase in N inputs caused by increased cultivation of N fixing crops such as alfalfa (Medicago sativa L.), soybean [Glycine max (L.) Merr.], and other legumes. These crops have a much greater capacity to fix atmospheric N than natural vegetation. For example, alfalfa can fix approximately 218 kg N ha -1 yr -1 , whereas deciduous forests can fix only about 12 kg N ha -1 yr -1 (Keeney, 1979;Jordan and Weller, 1996). In some agro-ecosystems, legume fixation may even be the largest source of N. For example, Keeney (1979) estimated that for the state of Wisconsin, 2 N fixation by legumes exceeded inputs from commercial fertilizer by 2.5 times. Peterson and Russelle (1991) estimated that for the eight states of the U.S. Corn Belt, alfalfa alone contributed 1 × 10 9 kg of N compared with 4 × 10 9 kg from commercial fertilizer. It is important to consider this N source because it may contribute NO 3 -to ground water and surface waters either directly through N added to the soil or indirectly as the result of mineralization of plant re...