“…Poultry litter also contains heavy metals, such as As, Cd, Co, Cr, Cu, Ni, Pb, and Zn (12,19,26,30,31), obligate and opportunistic pathogens, such as Yersinia enterocolitica, Listeria monocytogenes, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa, enteropathogenic Escherichia coli strains such as O157:H7, Campylobacter jejuni, and Clostridium perfringens, and fecal indicator bacteria (FIB), such as E. coli and enterococci (21,28). Studies have shown these metals and bacteria can be transported from agricultural fields on which litter has been applied to receiving waters (3,29,31,44), and quantities can exceed 56% of applied NH 3 -N and 10% of applied total phosphorus (11,12,15,20). Furthermore, E. coli in runoff from pastures with broadcast-applied litter can exceed 1,300 CFU 100 ml Ϫ1 (43).…”