Nutrient and Phytoplankton Analysis of a Mediterranean Coastal Area

Sebastià, M. T.; Rodilla, M.

doi:10.1007/s00267-012-9986-3

Cited by 17 publications

(13 citation statements)

References 33 publications

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“…According to our literature survey, HPLC-CHEMTAX results were not throughly compared with carbon biomass of phytoplankton groups in the Mediterranean Sea with a time series investigation. Previous studies focuses on impact of nutrients on phytoplankton composition and abundance mainly based on CHEMTAX results disregarding phytoplankton carbon biomass [24, 65, 66, 67]. Our results show that CHEMTAX assigned phytoplankton groups should not be assumed as a proxy to abundance or carbon biomass of diatoms, dinoflagellates and haptophytes in the study region located in the northeastern Mediterranean coast.…”

Section: Discussionmentioning

confidence: 63%

Microalgal pigments and their relation with phytoplankton carbon biomass on the northeastern Mediterranean Sea shore, with special emphasis on nanophytoplankton

Eker-Develi

Konucu

Örek

et al. 2019

Preprint

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Marker pigments are used as a proxy for biomass of distinct phytoplankton classes in 15 different oceanic regions. However, sometimes disagreements are observed between microscopy and 16 accessory-pigment based approaches in distinct regions mainly due to changing environmental factors 17 governing diversity and structure of community composition. In this study, concordance between 18 microscopy and HPLC-CHEMTAX methods were investigated first time in coastal waters of Erdemli, 19 Turkey, in the Levantin Basin of the northeastern Mediterranean Sea by weekly intervals during 2015-20 2016. According to our results, marker pigment of diatoms, fucoxanthin, which was the most 21 prominent pigment in the study area during most of the year, was a better indicator of diatom 22 abundance than diatom carbon biomass. CHEMTAX derived values of diatom chlorophyll a (Chl a) 23 were not in concert with either abundance or carbon biomass of this group. Contribution of 24 dinoflagellates and cryptophytes to the phytoplankton community was underestimated with pigment 25 based approach. Accessory pigment of cyanophytes, zeaxanthin, was also an important pigment in the 26 samples. Biomass of haptophytes seemed to be overestimated by HPLC-CHEMTAX analysis. In 27 contrast to diatoms, CHEMTAX derived chlorophyll a values of cryptophytes were correlated with 28 abundance of this group but not with alloxanthin. Inclusion of live counts of nanoplanktic 29 cryptophytes, haptophytes and prasinophytes provided a better correlation between microscopy and 30 pigment based results. According to CHEMTAX analysis, nanoplankton and picoplankton constituted 31~55% of Chl a in the region.32 33 Mediterranean Sea 4 [1, 2, 3]. Among these parameters phytoplankton cell counting and calculation of cell volume is a 5 process of extremely fatiguing and time consuming and not very suitable for analysis of high number 6 of samples. For estimating standing stock from Chl a values obtained either through satellites or direct 7 seawater measurements in a region, factors influencing C:Chl a ratios should be considered. This ratio 8 varies between <10 and >200 among different phytoplankton classes as well as with change in nutrient 9concentrations, temperature, irradiance, growth phases and from species to species [3, 4, 5, 6, 7, 8]. 10 Each of these parameters are variable in different seawater regions and should be validated with field 11 data in less-studied locations. Chl a content of each phytoplankton group can be estimated based on 12 their marker pigments determined by HPLC-CHEMTAX analysis and from these groups specific Chl 13 a values, carbon biomass of phytoplankton groups could be assessed. HPLC analysis is faster than 14 microscopy and provides one step analysis of all size groups through retention of pico-nano-and 15 micro-phytoplankton on filters [9, 10]. However, verification of results obtained from pigment based 16 approach by microscopy is a necessity at least for some of the samples due to sharing of some marker 17 pigments by different phytop...

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Section: Discussionmentioning

confidence: 63%

Microalgal pigments and their relation with phytoplankton carbon biomass on the northeastern Mediterranean Sea shore, with special emphasis on nanophytoplankton

Eker-Develi

Konucu

Örek

et al. 2019

Preprint

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“…The DSi concentration in all tanks were lower than that in raw water (Table 1). Studies found that groundwater is characterized by high silica content, because of the lixiviation of biogenic silica from Gramineae (Sebastia & Rodilla, 2013;Sebastiá et al, 2012;Sospedra et al, 2018). The aquaculture water was the mixture of seawater and underground brine in this study.…”

Section: Effects Of Water Quality Parameters On the Phytoplankton Cmentioning

confidence: 83%

Phytoplankton community structure and diversity in the indoor industrial aquaculture system for Litopenaeus vannamei revealed by high‐throughput sequencing and morphological identification

Qiao

Chang

et al. 2019

Aquac Res

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The explosive multiplication of phytoplankton caused by water eutrophication often occur in the intensive shrimp aquaculture. To comprehensively assess the diversity and community structure of phytoplankton in the waters of typical indoor industrial aquaculture system for Litopenaeus vannamei, a combination of high‐throughput sequencing and morphological identification methods were used in the present study. A total of 41 genera belong to nine phyla were detected by both methods. Chlorophyta, Cyanophyta and Bacillariophyta were found to be three dominant phyla. The high‐throughput sequencing revealed that green algae and cyanobacteria were the most dominant phytoplankton; however, diatoms were the first dominant phytoplankton by using the morphological identification. At the genus level, Picochlorum and Synechococcus were dominant, accounting for 20.94%–97.19% and 0.01%–52.81% of total phytoplankton, revealed by the high‐throughput sequencing. Therefore, more attention should be paid to their ecological impacts on the surrounding sea areas or potential toxicity to shrimp. Cyclotella was the most dominant genus revealed by the morphological identification. High‐throughput sequencing revealed a high diversity and small‐sized phytoplankton which were undetected by microscopy. Both methods provide similar information on the environmental drivers of phytoplankton community. NO3−, NH4+, DIP, DSi, DON and DOP concentrations were the main factors influencing the phytoplankton community structure and diversity.

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“…The decrease or depletion of silica usually causes the decline of diatoms and proliferations of cyanobacteria (Casé et al 2008). In this experiment, the tanks were filled with coastal waters from Gandia Harbor characterized by high silica levels (Sebastiá et al 2012;Sebastiá and Rodilla 2013).…”

Section: Resultsmentioning

confidence: 99%

“…comm. ) to obtain the contribution to total chlorophyll a (Chla) from the phytoplankton and periphyton groups identified with microscopy as described in Sebastiá et al (2012) and Sebastiá and Rodilla (2013). In order to identify groups of samples with similar characteristics, a cluster analysis was performed using STATGRAPHICS Centurion XVI.I to group samples according to pigments concentration.…”

Section: Biological Parametersmentioning

confidence: 99%

Periphyton and phytoplankton assessment in a shrimp nursery: signature pigments analysis

Llario

Rodilla

Falco

et al. 2019

Int. J. Environ. Sci. Technol.

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Microalgae play a fundamental role in aquaculture systems, especially in nurseries. They can supplement the nutrition of cultured species, and also contribute to keep good water quality. Their value depends on the groups present in major abundance. Thus, being able to understand the factors that influence microalgae composition is key for an improved system management. In this study, the microalgae community structure in a Marsupenaeus japonicus nursery is studied. Instead of the classic microscopy approach, the composition and abundance of periphyton and phytoplankton were analyzed using signature pigments analysis by high pressure liquid chromatography coupled with the software CHEMTAX. The environmental parameters, which could affect periphyton and phytoplankton groups, were analyzed statistically. The results showed that diatoms were the dominant group both in phytoplankton and periphyton. The analysis of signature pigments, allowed to report the presence of previously undetected groups on periphyton, prasinophytes and prymnesiophytes, which are characterized by a high nutritional value. This is especially important in nurseries because of shrimp grazing on periphyton can increase post-larvae survival. Nutrients played a key role on phytoplankton development, but had a minor effect on periphyton, which was more affected by colonization processes and other environmental variables.

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Nutrient and Phytoplankton Analysis of a Mediterranean Coastal Area

Cited by 17 publications

References 33 publications

Microalgal pigments and their relation with phytoplankton carbon biomass on the northeastern Mediterranean Sea shore, with special emphasis on nanophytoplankton

Microalgal pigments and their relation with phytoplankton carbon biomass on the northeastern Mediterranean Sea shore, with special emphasis on nanophytoplankton

Phytoplankton community structure and diversity in the indoor industrial aquaculture system for Litopenaeus vannamei revealed by high‐throughput sequencing and morphological identification

Periphyton and phytoplankton assessment in a shrimp nursery: signature pigments analysis

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