Nutrient and other environmental influences upon rumen protozoal growth and metabolism

Abstract

Methods and procedures 20 Results and discussion.. . • 26 In Vitro Volatile Fatty Acid Production by Rumen Protozoa ' 29 Responses of rumen protozoa to pH changes and time of in vitro fermentations 31 Methods and procedures 31 Results and discussion 37 Responses of rumen protozoa to substrate and protozoal concentration changes of in vitro fermentations 41 Methods and procedures 42 Results and discussion 44 Responses of rumen protozoa bo diethylstilbestrol and antibiotics of in vitro fermenta tions. 48 Methods and procedures 49 Results and discussion 51 Responses of rumen protozoa to sodium, potas sium and phosphorus of in vitro fermentations 57 Methods and procedures 58 Results and discussion 63 CONCLUSIONS AND SUMMARY ' 78 ill Page PART II. PRODUCTION OF VOLATILE FATTY ACIDS AND AMMONIA IN THE RUMEN OF FAUNATED •AND PROTOZOA-FREE LAMBS 82a REVIEW OF LITERATURE 82b Techniques for Obtaining Protozoal-Free Lambs.. .. 82b In Vivo Production-of Rumen Volatile Fatty Acids and Ammonia in Faunated and Protozoal-Free Lambs. 89 EXPERIMENTAL PROCEDURE AND RESULTS 98 Rearing Isolated NewBorn Orphan Lambs 98 Methods and procedures ' 98 Results and discussion 105 Def'aunating the Rumen of Lambs Having Protozoal Populations Methods and results Discussion Volatile Fatty Acid Production in the Rumen of Faunated, Partially Faunated and Protozoal-Free Lambs Methods and procedures.. Results and discussion.. .. Ruminai Ammonia Production in the Rumen of Faunated, Partially Faunated and Protozoal-Free Lambs.. .. 138 Methods and procedures 139 Results and discussion.. .