Nutrient amendments affect Trichoderma atroviride conidium production, germination and bioactivity

Effects of temperature, light and incubation period on production, germination and bioactivity ofTrichoderma atroviride

“…This study was part of a programme aimed to develop new knowledge of effects of growth conditions, to increase persistence and efficiency of the key agent T. atroviride LU132, which has been commercialized and to understand the factors which may influence conidium fitness (quantity and quality) for biological control formulations of conidia that are robust (long‐surviving) and active against target plant pathogens (Daryaei ; Daryaei et al . ,b,c,d). Although the effects of culture conditions on the quantity of conidia produced of this strain have been previously studied (Steyaert et al .…”

Culturing conditions affect biological control activity ofTrichoderma atrovirideagainstRhizoctonia solaniin ryegrass

“…Sucrose as a hydrocarbon source during T. atroviride LU132 conidium production has been shown to result in superior conidium fitness (quantity and quality), particularly enhancing conidium survival and bioactivity after storage (Daryaei et al . ,d). However, in the present study, 21 days after sowing, conidia produced at 20°C gave greater rhizosphere colonization than conidia produced at 30°C, and then within each conidium production temperature (20°C or 30°C), conidia produced with dextrose (20°C‐Dex or 30°C‐Dex) gave greater colonization than those produced using sucrose.…”

“…Similarly, previous studies have shown that T. atroviride LU132 outcompeted R. solani on dual plate assays (Daryaei et al . ,b,c,d,e). It has been suggested that Trichoderma metabolites contribute fungistatically to supress the growth and development of R. solani in soil or in agar dual cultures, which are proposed to be differentially modulated by NADPH oxidase (Dr Artemio Mendoza‐Mendoza 2014; personal communication).…”

“…Previous studies by Daryaei et al . (,d) have shown that the growth medium with C : N ratio treatment of 5 : 1 gave high conidium production, germination and bioactivity for T. atroviride LU132 conidia, and also stability and bioactivity after storage. However, high concentrations of carbon caused catabolite repression affecting conidium production.…”

“…atroviride LU132 were studied in agar plates (Daryaei et al . ,d,e). Fresh or dry conidia obtained from different culture‐growing conditions were also assessed (during 6 months of storage) for quantities of conidia produced and their quality, as indicated by germination and subsequent bioactivity against R. solani (see Daryaei et al .…”