Nutrient absorption by Aphidius ervi larvae

Caccia, Silvia; Leonardi, M.G.; Grimaldi, Annalisa; Eguileor, Magda de; Pennacchio, Francesco; Giordana, B.

doi:10.1016/j.jinsphys.2005.06.010

Cited by 30 publications

(37 citation statements)

References 21 publications

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“…Larval endophagous parasitoids of aphids, such as A. ervi, feed on insect host haemolymph via ingestion and trans-cuticle absorption (Pennacchio et al 1995;Rahbé et al 2002;Giordana et al 2003;Caccia et al 2005). As in animals, generally parasitoids do not encode pathways for essential amino acid biosynthesis and must obtain these amino acids from their diet, particularly in the form of free amino acids during early larval development (Thompson 1986).…”

Section: Amino Acid Biosynthesis and Metabolismmentioning

confidence: 99%

“…As in animals, generally parasitoids do not encode pathways for essential amino acid biosynthesis and must obtain these amino acids from their diet, particularly in the form of free amino acids during early larval development (Thompson 1986). In A. ervi, external sources of essential and nonessential free amino acids play a substantial role in early larval nutrition (Giordana et al 2003;Caccia et al 2005). Because an adequate supply of free amino acids in aphid haemolymph is required for successful A. ervi development and survival (Pennacchio et al 1999;Rahbé et al 2002), competition for free amino acids by R. insecticola 5.15 is a potential factor affecting parasitoid development.…”

Section: Amino Acid Biosynthesis and Metabolismmentioning

confidence: 99%

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Genomic basis of endosymbiont-conferred protection against an insect parasitoid

Hansen¹,

Vorburger²,

Moran³

2011

Genome Res.

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Bacterial endosymbionts exert a variety of beneficial effects on insect hosts. In pea aphids (Acyrthosiphon pisum), several inherited endosymbiont species protect their hosts against parasitoid wasps, which are major natural enemies. However, strains of these symbiont species vary in their ability to confer protection against parasitoids, with some conferring almost complete protection and others conferring almost none. In this study, two strains of the endosymbiont Regiella insecticola (R. insecticola 5.15 and R. insecticola LSR1) were found to differ in ability to protect pea aphids attacked by the parasitoid Aphidius ervi. Parasitism trials reveal that R. insecticola 5.15, but not R. insecticola LSR1, significantly reduced parasitoid success and increased aphid survivorship. To address the potential genetic basis of protection conferred by R. insecticola 5.15 we sequenced the genome of this symbiont strain, and then compared its gene repertoire with that of the already sequenced nonprotective strain R. insecticola LSR1. We identified striking differences in gene sets related to eukaryote pathogenicity. The protective strain R. insecticola 5.15 encoded five categories of pathogenicity factors that were missing or inactivated in R. insecticola LSR1. These included genes encoding the O-antigen biosynthetic pathway, an intact Type 1 Secretion System and its secreted RTX toxins, an intact SPI-1 Type 3 Secretion System and its effectors, hemin transport, and the twocomponent system PhoPQ. These five pathogenicity factors and translocation systems are hypothesized to collectively play key roles in the endosymbiont's virulence against parasitoids, resulting in aphid protection. Mechanisms through which these factors may target parasitoids are discussed.

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Section: Amino Acid Biosynthesis and Metabolismmentioning

confidence: 99%

Section: Amino Acid Biosynthesis and Metabolismmentioning

confidence: 99%

Genomic basis of endosymbiont-conferred protection against an insect parasitoid

Hansen¹,

Vorburger²,

Moran³

2011

Genome Res.

View full text Add to dashboard Cite

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“…Early studies with insect gut suggested that sugars were absorbed totally by diffusion without involving carrier proteins in the process (Treherne, 1957;Treherne, 1958). More recently, several studies involving both physiology and molecular biology have described a number of sugar transporters of the GLUT family in insects from several different orders (Caccia et al, 2005;Chen et al, 2006;Escher and RasmusonLestander, 1999;Pascual et al, 2006;Wang and Wang, 1993). The first report describing a detailed model for carrier-mediated sugar movement across an absorbing insect epithelium was published in 2007 (Caccia et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel sodium-dependent fructose transport activity in the hepatopancreas of the Atlantic lobsterHomarus americanus

Sterling

Cheeseman

Ahearn

2009

Journal of Experimental Biology

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“…Earlier studies documented the active absorption of essential amino acids in mosquitoes (Uchida et al, 2003;Uchida et al, 2001;Uchida et al, 1990) and other insects (Caccia et al, 2005;Castagna et al, 1997;Giordana et al, 1989;Nedergaard, 1972;Wolfersberger, 2000). Using genome data mining in combination with comparative phylogenetic analysis of transporters in selected organisms with published genomes, we identified and compared key families of secondary transporters that contribute to the amino acid traffic network in metazoans (Boudko et al, 2005c).…”

Section: Introductionmentioning

confidence: 99%

Synergy and specificity of two Na+–aromatic amino acid symporters in the model alimentary canal of mosquito larvae

Okech

Meleshkevitch

Miller

et al. 2008

Journal of Experimental Biology

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SUMMARYThe nutrient amino acid transporter (NAT) subfamily is the largest subdivision of the sodium neurotransmitter symporter family (SNF; also known as SLC6; HUGO). There are seven members of the NAT population in the African malaria mosquito Anopheles gambiae, two of which, AgNAT6 and AgNAT8, preferably transport indole-and phenyl-branched substrates, respectively. The relative expression and distribution of these aromatic NATs were examined with transporter-specific antibodies in Xenopus oocytes and mosquito larval alimentary canal, representing heterologous and tissue expression systems, respectively. NATspecific aromatic-substrate-induced currents strongly corresponded with specific accumulation of both transporters in the plasma membrane of oocytes. Immunolabeling revealed elevated expressions of both transporters in specific regions of the larval alimentary canal, including salivary glands, cardia, gastric caeca, posterior midgut and Malpighian tubules. Differences in relative expression densities and spatial distribution of the transporters were prominent in virtually all of these regions, suggesting unique profiles of the aromatic amino acid absorption. For the first time reversal of the location of a transporter between apical and basal membranes was identified in posterior and anterior epithelial domains corresponding with secretory and absorptive epithelial functions, respectively. Both aromatic NATs formed putative homodimers in the larval gut whereas functional monomers were over-expressed heterologously in Xenopus oocytes. The results unequivocally suggest functional synergy between substrate-specific AgNAT6 and AgNAT8 in intracellular absorption of aromatic amino acids. More broadly, they suggest that the specific selectivity, regional expression and polarized membrane docking of NATs represent key adaptive traits shaping functional patterns of essential amino acid absorption in the metazoan alimentary canal and other tissues.

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Nutrient absorption by Aphidius ervi larvae

Cited by 30 publications

References 21 publications

Genomic basis of endosymbiont-conferred protection against an insect parasitoid

Genomic basis of endosymbiont-conferred protection against an insect parasitoid

Identification of a novel sodium-dependent fructose transport activity in the hepatopancreas of the Atlantic lobsterHomarus americanus

Synergy and specificity of two Na+–aromatic amino acid symporters in the model alimentary canal of mosquito larvae

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