Nutlin-Loaded Magnetic Solid Lipid Nanoparticles for Targeted Glioblastoma Treatment

Abstract

Aim Glioblastoma multiforme is one of the deadliest forms of cancer, and current treatments are limited to palliative cares. The present study proposes a nanotechnology-based solution able to improve both drug efficacy and its delivery efficiency. Materials & methods Nutlin-3a and superparamagnetic nanoparticles were encapsulated in solid lipid nanoparticles, and the obtained nanovectors (nutlin-loaded magnetic solid lipid nanoparticle [Nut-Mag-SLNs]) were characterized by analyzing both their physicochemical properties and their effects on U-87 MG glioblastoma cells. Results Nut-Mag-SLNs showed good colloidal stability, the ability to cross an in vitro blood–brain barrier model, and a superior pro-apoptotic activity toward glioblastoma cells with respect to the free drug. Conclusion Nut-Mag-SLNs represent a promising multifunctional nanoplatform for the treatment of glioblastoma multiforme.

Antioxidants and Nanotechnology: Promises and Limits of Potentially Disruptive Approaches in the Treatment of Central Nervous System Diseases

“…For example, superparamagnetic iron oxide nanoparticles (SPIONs) interact with external alternated magnetic fields and, therefore, they can be used both for hyperthermia and as contrast agents in magnetic resonance imaging (MRI) . Moreover, the application of an external magnetic field can be exploited to favor the crossing of the BBB of SPIONs, as recently demonstrated . Iron oxide nanoparticles functionalized with catalase were shown to be able to convert ROS into O 2 mitigating hypoxia effects in tumor microenvironment.…”

Cell Membrane‐Coated Magnetic Nanocubes with a Homotypic Targeting Ability Increase Intracellular Temperature due to ROS Scavenging and Act as a Versatile Theranostic System for Glioblastoma Multiforme

“…We further studied this accumulation by using a theoretical numerical model that was previously described by our group. [14]…”

“…To assess the ability to guide the CM-NCubes across the BBB and towards the glioblastoma site using a static magnetic field, an in vitro fluidic model of the BBB [14] was used. The fluidic model consisted of two parallel channels (top and bottom channel) that were separated by a porous membrane, derived from 6 well falcon transwell inserts (3 µm pore size).…”

“…Aiming at fully exploiting the magnetic component of the CM-NCubes, we assessed the combinational effect of a static magnetic field in close proximity to a dynamic microfluidic BBB in vitro model that consisted of an upper chamber seeded with bEnd.3 cells and a lower static compartment seeded with U-251 MG cells. [14] The results of this experiment demonstrated that under the effect of the static magnetic field the internalization and the subsequent crossing of the CM-NCubes through the bEnd.3 cell layer is significantly faster.…”

Enhanced In Vitro Magnetic Cell Targeting of Doxorubicin-Loaded Magnetic Liposomes for Localized Cancer Therapy

“…We also studied the aforementioned magnetic targeting problem using the numerical model that was introduced in literature [ 53 ]. The considered flow channel had a rectangular cross-section (3.8 mm width, 0.4 mm height) and the flow was uniform (thus considered to be fully developed) over a 17 mm span.…”