Nut Allergen Detection

Fielder, Richard; Higgs, Warren; Barden, Katie

doi:10.1002/9780470637685.ch20

Cited by 1 publication

(4 citation statements)

References 24 publications

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“…Numerous kits are commercially available (Poms et al, 2004a;Schubert-Ullrich et al, 2009;Fielder et al, 2010). All tests provide quantitative results, based on in-house reference materials, and present variable cross-reactivity among nuts.…”

Section: Immunological Methodsmentioning

confidence: 99%

“…Matrix effects and food processing may also affect the detection of nut allergens by ELISA (Garber and Perry, 2010). Lateral flow devices and dipsticks are also commercially available and mostly used for screening purposes (Schubert-Ullrich et al, 2009;Fielder et al, 2010).…”

Section: Immunological Methodsmentioning

confidence: 99%

“…The reported LODs for ELISA kits available on the market range from 0.1 mg/kg for Ara h 1, to 0.5 mg/kg for Ara h 2 and 2.5 mg/kg for peanut proteins. The LOQs vary in the range of 1 to 20 and 1 to 15 mg/kg for Ara h 1 and Ara h 2, respectively, and of 3.3 to 90 mg/kg for peanut proteins (Fielder et al, 2010).…”

Section: Detection Of Allergens and Allergenic Ingredients In Foodmentioning

confidence: 99%

“…Immunological methods 18.6.1.1. ELISA Many ELISA methods for the detection and quantification of peanut in foods are commercially available (Fielder et al, 2010;Zeleny and Schimmel, 2010). Some detect total soluble proteins or a mixture of proteins, whereas others are designed to target a specific peanut allergen (Ara h 1 or Ara h 2) (Schmitt et al, 2004).…”

Section: Detection Of Allergens and Allergenic Ingredients In Foodmentioning

confidence: 99%

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Scientific Opinion on the evaluation of allergenic foods and food ingredients for labelling purposes

Products¹

2014

EFS2

115

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Following a request from the Food Safety Authority of Ireland, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA Panel) was asked to deliver a scientific opinion on the evaluation of allergenic foods and food ingredients for labelling purposes. In view of the request, the NDA Panel decided to update its previous opinions relative to food ingredients or substances with known allergenic potential listed in Annex IIIa of 2003/89/EC, as amended. These include cereals containing gluten, milk and dairy products, eggs, nuts, peanuts, soy, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites. The opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. It includes information on the prevalence of food allergy in unselected populations, proteins identified as food allergens, cross-reactivities, the effects of food processing on the allergenicity of foods and ingredients, methods for the detection of allergens and allergenic foods, doses observed to trigger adverse reactions in sensitive individuals and risk assessment methodologies that have been used to derive individual and population thresholds for selected allergenic foods. The present opinion relates to immunoglobulin (Ig)E-and non-IgE-mediated food allergy, to coeliac disease and to adverse reactions to sulphites in food, and it does not address non-immune-mediated adverse reactions to food. For each food ingredient or substance listed in Annex IIIa, it includes information on the prevalence of food allergy in unselected populations, on proteins identified as food allergens, on cross-reactivities, on the effects of food processing on the allergenicity of foods and ingredients, on methods for the detection of allergens and allergenic foods, and on doses observed to trigger adverse reactions in sensitive individuals.Immune-mediated adverse reactions to foods manifest with clinical signs and symptoms of variable severity and duration, which may affect different organs and systems. Anaphylactic reactions to food are IgE mediated and may occur at any age. Non-IgE-mediated food allergy includes a wide range of diseases, including protein-induced enterocolitis and eosinophilic oesophagitis.A careful family and clinical history are the basis for diagnosis of food allergy. Food diaries, skin prick tests (SPTs), allergen-specific IgE measurements, food elimination diets and food challenges are part of the standard protocol for the diagnosis of food allergy. A positive SPT indicates sensitisation to the tested food, but it is not diagnostic of food allergy. Allergen-specific serum IgE antibodies similarly denote sensitisation to a particular food, but they are not diagnostic without a clinical history or food challenge. The use of atopy patch tests for the diagnosis of food allergy is controversial. Other available tests have no current role in the diagnosis of food allergy. Diag...

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Section: Immunological Methodsmentioning

confidence: 99%