Objective: This study was conducted to determine the interventions of nurses working in intensive care units to prevent ventilator-associated pneumonia (VAP) in intensive care units (ICUs). Methods: This study was a descriptive study. Nurses working in the adult ICUs of six state and four university hospitals within the boundaries of the municipality of Ankara were participated in this study. The research sample included 290 ICU nurses. In the study, data were collected by questionnaire developed by researchers. For the statistical evaluation of the data, Student's t-test was used to compare two groups, whereas analysis of variance was used to compare more than two groups. Results: Although the number of the nurses who sterilized their hands with alcohol-containing liquids or measured the cuff pressure to prevent VAP was found to be below average, the number of nurses who preferred alternative methods appeared to be above average. Nurses with a university degree who worked in university hospitals and surgical ICUs implemented methods to prevent VAP more successfully, and the difference between the groups was statistically significant (p < 0.05). Conclusion: None of the hospitals had a protocol regarding the prevention of VAP, and only 7.9% of the nurses said that they read and followed the scientific literature on the issue. This study suggested that institutions should develop their own protocols regarding the prevention of VAP, nurses should be referred to training programs to enhance their knowledge on the issue, and VAP prevention methods should be regularly monitored.