Numerous nerves with calcitonin gene-related peptide-like immunoreactivity innervate junctional epithelium of rats

Byers, Margaret R.; Mecifi, K B; Kimberly, Charles L.

doi:10.1016/0006-8993(87)90598-1

Cited by 44 publications

(23 citation statements)

References 10 publications

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“…2 Another unique feature of the dental pulp innervation is the high density of exclusively nociceptive primary afferents. [59][60][61] Further, the innervation of the dental pulp is gained and lost as deciduous teeth exfoliate and permanent teeth erupt. Thus, there is a very dynamic pattern of axonal targeting through the shift from primary (deciduous) to permanent dentition, and during the maturation of each of these sets of teeth.…”

Section: Discussionmentioning

confidence: 99%

Stem Cells of the Apical Papilla Regulate Trigeminal Neurite Outgrowth and Targeting through a BDNF-Dependent Mechanism

Almeida

Chen

Henry

et al. 2014

Tissue Engineering Part A

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Regenerative endodontic procedures have become a valuable alternative for the treatment of immature teeth with pulp necrosis. In addition to resolution of periradicular pathosis and promotion of continued root development, positive vitality testing has been observed in some regenerative clinical cases. Importantly, the positive response to electric stimulation of the regenerated tissue requires targeting of periradicular axons into the previously empty root canal space. However, the mechanism by which this process occurs is largely unknown. Since stem cells of the apical papilla (SCAP) have been proposed to populate the root canal following regenerative endodontic procedures, we hypothesized that SCAP regulate neurite outgrowth and axonal targeting. To test this hypothesis, we established primary co-cultures of human SCAP and rat trigeminal neurons, and performed neurite outgrowth assays using ELISA and confocal microscopy to determine the effect of increasing concentration of SCAP on the total neurite outgrowth and axonal targeting. In addition, we evaluated whether SCAP evoked axonal targeting in vivo using a matrigel subcutaneous implant assay. Data were analyzed by ANOVA with Bonferroni's post hoc test, and significance was set at p < 0.05. The results demonstrated that SCAP release a soluble factor that regulates neurite outgrowth from cultured trigeminal neurons. Next, we demonstrated that this effect is completely abolished by pretreatment with a neutralizing antibody to brain-derived neurotrophic factor (BDNF), but not by antibodies to other neurotrophins. Further, SCAP release BDNF in a concentration-dependent manner as detected by ELISA, and trigger directed axonal targeting both in vitro and in vivo as demonstrated by microfluidic and matrigel implant experiments, respectively. Collectively, these results suggest that SCAP may be responsible for the chemical signal driving axons to target regenerated tissue via a BDNF-dependent mechanism.

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Section: Discussionmentioning

confidence: 99%

Stem Cells of the Apical Papilla Regulate Trigeminal Neurite Outgrowth and Targeting through a BDNF-Dependent Mechanism

Almeida

Chen

Henry

et al. 2014

Tissue Engineering Part A

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“…Substance P (SP), a sensory neuropeptide released from the peripheral endings of sensory nerves during infl ammation, can modify the secretion of pro-infl ammation cytokines from immunocompetent cells. A number of different neuropeptides, including SP, are known to be present in the nerve fi bers of dental pulp and the periodontal tissues in rats, cats, monkeys, and humans [1][2][3][4][5][6]. Further, SP-immunopositive nerve fi bers have been shown to change their patterns as a result of local pulp trauma possibly indicating that SP-containing fi bers take part in the infl ammatory process in connection with tissue injury and repair [7].…”

Section: Introductionmentioning

confidence: 99%

Substance P stimulates release of RANKL via COX-2 expression in human dental pulp cells

2006

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“…A number of different neuropeptides, including CGRP and SP, are known to be present in the nerve fibers that supply tooth pulp and the periodontium in rats, cats, monkeys and humans (Olgart et al, 1977;Wakisaka et al, 1985;Silverman and Kruger 1987;Byers et al, 1987;Luthman et al, 1988;Casasco et al, 1990). Further, the morphology and distribution of CGRP and SP through immunoreactive nerves have been shown to change their pattern as a result of local pulp trauma, which may indicate that CGRP and SP-containing fibers take part in the inflammatory process in connection with tissue injury and repair (Kvinnsland and Heyeraas, 1992).…”

Section: Introductionmentioning

confidence: 99%

Neuropeptides stimulate production of interleukin-1�, interleukin-6, and tumor necrosis factor-a in human dental pulp cells

Yamaguchi

Kojima

Kanekawa

et al. 2004

Inflammation Research

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Numerous nerves with calcitonin gene-related peptide-like immunoreactivity innervate junctional epithelium of rats

Cited by 44 publications

References 10 publications

Stem Cells of the Apical Papilla Regulate Trigeminal Neurite Outgrowth and Targeting through a BDNF-Dependent Mechanism

Stem Cells of the Apical Papilla Regulate Trigeminal Neurite Outgrowth and Targeting through a BDNF-Dependent Mechanism

Substance P stimulates release of RANKL via COX-2 expression in human dental pulp cells

Neuropeptides stimulate production of interleukin-1�, interleukin-6, and tumor necrosis factor-a in human dental pulp cells

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