Optical fiber connectors (OFCs) have become widely used in a variety of fields as optical fiber communication technology has developed. Compared with the other working environment of the OFCs, the working environment in the airplanes is more complex and harsher, which leads to an increase in connector failure rate and failure impact. In this paper, based on the analysis of the influence of vibration, high-low temperature cycle, and oceanic atmosphere in the aircraft working environment on the physical parameters of OFCs, the effects of the complex environment in the airplane service on insertion loss of OFCs is studied and discussed.