Incorporation of the glucose in presence of phosphate and acetate buffer were studied to investigate the poly(N-methylpyrrole)/polyvinyl sulfonic acid/sodium nitrate/glucose oxidase (P(NMP)/PVS/NaNO 3 /GODx) electrode. The P(NMP)/PVS/NaNO 3 films were electrochemically synthesized and characterized by using electrochemical technique, FTIR and SEM. Glucose oxidase was immobilized by cross-linking via glutaraldehyde on the galvanostatically synthesized poly(N-methylpyrrole) /polyvinyl sulfonic acid/sodium nitrate (P(NMP)/PVS/NaNO 3 ) film. The higher sensitivity of P(NMP)/PVS/NaNO 3 /GODx electrode was recorded in phosphate buffer than that of acetate buffer and that was found to be 4 µA/mM and 2.2 µA/mM, respectively. The kinetics parameters of P(NMP)/PVS/NaNO 3 /GODx electrode were determined for the phosphate buffer and acetate buffer. In phosphate buffer the observed values of K m and I max were 14.4 mM and 111.2 µA, respectively and in acetate buffer 16.9 mM and 68 µA, respectively. The stability of synthesized P(NMP)/PVS/NaNO 3 /GODx electrode in phosphate buffer was found to be more than that of acetate buffer.