SUMMARYDirect numerical simulations based on finite-difference solutions of the unsteady Navier-Stokes equations are used for investigating two-dimensional laminar separation bubbles in incompressible boundary-layer flows over smooth backward-facing steps. An attempt has been made to clarify the mechanism of natural unsteadiness of the separation bubble that appears if the step height exceeds a certain limit. It was found that this phenomenon is caused by hydrodynamic instability of the separated boundary layer.