Numerical Model of Electrokinetic Flow for Capillary Electrophoresis

Hu, Lianguang; Harrison, Jed; Masliyah, Jacob H.

doi:10.1006/jcis.1999.6250

Cited by 75 publications

(67 citation statements)

References 20 publications

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“…Burgreen & Nakache 1964;Hu et al 1999;Yang et al 2001;Chai & Shi 2007) as well as experimentally (e.g. Herr et al 2000;Pikal 2001;Horiuchi et al 2007;Kim et al 2008), attention has been chiefly confined to simple Newtonian fluids.…”

Section: Introductionmentioning

confidence: 99%

Steady electro-osmotic flow of a micropolar fluid in a microchannel

Siddiqui

Lakhtakia

2008

Proc. R. Soc. A.

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We have formulated and solved the boundary-value problem of steady, symmetric and onedimensional electro-osmotic flow of a micropolar fluid in a uniform rectangular microchannel, under the action of a uniform applied electric field. The Helmholtz-Smoluchowski equation and velocity for micropolar fluids have also been formulated. Numerical solutions turn out to be virtually identical to the analytic solutions obtained after using the Debye-Hückel approximation, when the microchannel height exceeds the Debye length, provided that the zeta potential is sufficiently small in magnitude. For a fixed Debye length, the mid-channel fluid speed is linearly proportional to the microchannel height when the fluid is micropolar, but not when the fluid is simple Newtonian. The stress and the microrotation are dominant at and in the vicinity of the microchannel walls, regardless of the microchannel height. The midchannel couple stress decreases, but the couple stress at the walls intensifies, as the microchannel height increases and the flow tends towards turbulence.

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Section: Introductionmentioning

confidence: 99%

Steady electro-osmotic flow of a micropolar fluid in a microchannel

Siddiqui

Lakhtakia

2008

Proc. R. Soc. A.

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“…However, during the dispensing step, the sample present in the loading channel can be carried away in the separation channel, which leads to dramatic band broadening, signal drift, and decrease in S/N [24,25,35,39]. This sample leakage has the same origin as the leakage previously presented with the Tinjection.…”

Section: Floating Modementioning

confidence: 83%

“…The control of the potential of the sample reservoir (S) can prevent from sample leakage during the dispensing step, generating an electrolyte flow from B to S which causes the push back of the sample from the channel junction (Fig. 1C) [21,35]. This process is simply called "sample pushback".…”

Section: T-type Injectionmentioning

confidence: 97%

“…This phenomenon is proportional to the intensity of the electric field applied in the separation channel and inversely proportional to the hydrodynamic resistance of the loading channel [35]. The control of the potential of the sample reservoir (S) can prevent from sample leakage during the dispensing step, generating an electrolyte flow from B to S which causes the push back of the sample from the channel junction (Fig.…”

Section: T-type Injectionmentioning

confidence: 99%

“…During the dispensing step, the electrolyte flow in the separation channel could induce a sample leakage [8,34,35]. This phenomenon is proportional to the intensity of the electric field applied in the separation channel and inversely proportional to the hydrodynamic resistance of the loading channel [35].…”

Section: T-type Injectionmentioning

confidence: 99%

See 2 more Smart Citations

Electrokinetic‐based injection modes for separative microsystems

2007

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Miniaturization of analytical instruments has attracted a wide interest in Analytical Chemistry over the past decade because of the advantages such as reduced reagent consumption and shorter analysis time. For chips involving separation, injection is a key step to achieve efficient and sensitive analysis. Electrokinetic injection mode is mostly used in chips because it is easier to generate flow motion in microsystems via electric potential control at channel extremities than pressure-driven flow. The injection step usually involves several intersecting channels. For each injection design, different injection modes can be done, depending on electric field sequences and distributions. This paper is an up-to-date review of these different modes on a chip.

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Integrated self-calibrationvia electrokinetic solvent proportioning for microfluidic immunoassays

Qiu

Harrison

2001

Electrophoresis

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On-board generation of a set of calibration standards was demonstrated within a microfluidic device designed to perform immunoassay. Electrokinetic flow was used to proportionally mix the antibody (Ab) to bovine serum albumin (BSA) and a diluting buffer, to provide varying Ab concentrations for downstream mixing with fluorescently labeled BSA (BSA*). Mixing ratios were determined from electrical impedance modeling of the fluidic network using P-SPICE software, and peak heights for the labeled species were analyzed relative to the concentration calculated from the model. For dilution and separation of fluorescently labeled amino acids, a linear calibration curve was obtained for mixing ratios of 0.118 to 7.46. A linear calibration curve was obtained for the immunoassay calibration using dilution ratios between 0.197 and 5.077. Deviations were observed at larger extremes, possibly due to leakage effects at intersections. Peak height reproducibility was +/- 3% for the immunoassay, using diluted monoclonal Ab in mouse ascites fluid as the analyte. Recovery for on-chip calibration was 92 +/- 6% versus calibrants prepared off-chip, indicating a small bias.

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Numerical Model of Electrokinetic Flow for Capillary Electrophoresis

Cited by 75 publications

References 20 publications

Steady electro-osmotic flow of a micropolar fluid in a microchannel

Steady electro-osmotic flow of a micropolar fluid in a microchannel

Electrokinetic‐based injection modes for separative microsystems

Integrated self-calibrationvia electrokinetic solvent proportioning for microfluidic immunoassays

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