Numerical investigation on improving photoacoustic imaging contrast based on temperature difference effect in biological tissues

Gong, Zhihao; Tao, Chao; Liu, Xiaojun; Deng, Mingxi

doi:10.1016/j.apacoust.2023.109585

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…PAM relies on optical absorption for imaging, enabling high-resolution visualization without the assistance of contrast agents [ 48 , 49 , 50 ]. Pulsed lasers induce transient thermal expansion on the target tissue leading to the generation of acoustic waves which are detected by an ultrasonic transducer and processed for the visualization of blood vascular networks and functional analysis of biological tissues [ 51 , 52 ]. High-speed wide-field photoacoustic imaging has been extensively utilized for the study of tumor microenvironments [ 53 , 54 , 55 , 56 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient Assessment of Tumor Vascular Shutdown by Photodynamic Therapy on Orthotopic Pancreatic Cancer Using High-Speed Wide-Field Waterproof Galvanometer Scanner Photoacoustic Microscopy

Lee,

Han,

Thapa Magar

et al. 2024

IJMS

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To identify the vascular alteration by photodynamic therapy (PDT), the utilization of high-resolution, high-speed, and wide-field photoacoustic microscopy (PAM) has gained enormous interest. The rapid changes in vasculature during PDT treatment and monitoring of tumor tissue activation in the orthotopic pancreatic cancer model have received limited attention in previous studies. Here, a fully two-axes waterproof galvanometer scanner-based photoacoustic microscopy (WGS-PAM) system was developed for in vivo monitoring of dynamic variations in micro blood vessels due to PDT in an orthotopic pancreatic cancer mouse model. The photosensitizer (PS), Chlorin e6 (Ce6), was utilized to activate antitumor reactions in response to the irradiation of a 660 nm light source. Microvasculatures of angiogenesis tissue were visualized on a 40 mm2 area using the WGS-PAM system at 30 min intervals for 3 h after the PDT treatment. The decline in vascular intensity was observed at 24.5% along with a 32.4% reduction of the vascular density at 3 h post-PDT by the analysis of PAM images. The anti-vascularization effect was also identified with fluorescent imaging. Moreover, Ce6-PDT increased apoptotic and necrotic markers while decreasing vascular endothelial growth factor (VEGF) expression in MIA PaCa-2 and BxPC-3 pancreatic cancer cell lines. The approach of the WGS-PAM system shows the potential to investigate PDT effects on the mechanism of angiographic dynamics with high-resolution wide-field imaging modalities.

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Section: Introductionmentioning

confidence: 99%

Efficient Assessment of Tumor Vascular Shutdown by Photodynamic Therapy on Orthotopic Pancreatic Cancer Using High-Speed Wide-Field Waterproof Galvanometer Scanner Photoacoustic Microscopy

Lee,

Han,

Thapa Magar

et al. 2024

IJMS

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Enhancing tissue imaging contrast in photoacoustic tomography using the ultrasound thermal effect

Gong,

Tao,

Liu

et al. 2024

Opt. Lett.

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Photoacoustic imaging is a powerful technique for obtaining high-resolution images of vascular distribution and physiological information about blood by utilizing the light absorption coefficient as an imaging contrast. However, visualizing weakly light-absorbing components without specific contrast agents or multi-wavelength techniques presents a challenge due to significant differences in light absorption between these components and blood. In this study, we propose a novel method that leverages the thermal effect of ultrasound to induce temperature differences and enhance the contrast of photoacoustic imaging. We conducted phantom experiments to verify the feasibility of our method. Our method effectively highlighted weakly light-absorbing components with strong acoustic absorption, even in the presence of highly light-absorbing components such as blood or melanin. Furthermore, it enabled the differentiation of components with similar light absorption but different acoustic absorption.

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Numerical investigation on improving photoacoustic imaging contrast based on temperature difference effect in biological tissues

Cited by 2 publications

References 41 publications

Efficient Assessment of Tumor Vascular Shutdown by Photodynamic Therapy on Orthotopic Pancreatic Cancer Using High-Speed Wide-Field Waterproof Galvanometer Scanner Photoacoustic Microscopy

Efficient Assessment of Tumor Vascular Shutdown by Photodynamic Therapy on Orthotopic Pancreatic Cancer Using High-Speed Wide-Field Waterproof Galvanometer Scanner Photoacoustic Microscopy

Enhancing tissue imaging contrast in photoacoustic tomography using the ultrasound thermal effect

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