Centrifugal pump impellers are high speed rotating components vulnerable to vibration resulting in the failure of the system pump eventually. While rotating, stresses are created due to the inertial forces. The model characteristics change due to the stress created and hence it is important to measure the model characteristics with consideration to the prestresses. Therefore, in this paper, the prestressed modal analysis of the impeller with blade thicknesses ranging from 3mm to 5mm with increments of 0.5mm, are analyzed by the numerical method, FEA for a rotational velocity of 3600rpm. The experimental modal testing of the impellers is then done using electro-dynamic shaker, to validate the FEA results.