Numerical dose-compensated in vitro fertilization inseminations yield high fertilization and pregnancy rates

Benoff, Susan; Cooper, George; Paine, T. D.; Hurley, Ian; Napolitano, Barbara; Jacob, Asha; Scholl, Gerald M.; Hershlag, Avner

doi:10.1016/s0015-0282(99)00136-3

Cited by 15 publications

(18 citation statements)

References 39 publications

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“…Note that expression of all biomarkers tested correlates with the rate of fertilization in IVF (58,60,67,69).…”

Section: Sperm Function Testingmentioning

confidence: 90%

“…One blood and two semen specimens were obtained from male partners of couples undergoing their first cycle of in vitro fertilization (n = 140) during the period February 1995 to August 1996. This population was based in the Long Island, NY, area and has been previously studied (38,(58)(59)(60)(61). Cadmium values for seminal plasma were averaged across each subject before examination of the relationship between mean cadmium and other parameters studied.…”

Section: Human Blood and Semen Specimensmentioning

confidence: 99%

“…Insemination policy was based on sperm concentration and motility according to World Health Organization (62) criteria and by acrosome morphology (58). Dose-compensated IVF inseminations were performed: the number of sperm used for each insemination was adjusted based on sperm number and acrosome morphology such that each oocyte was exposed to ≥25,000 sperm with normal acrosomes/mL (58).…”

Section: Human Blood and Semen Specimensmentioning

confidence: 99%

“…Dose-compensated IVF inseminations were performed: the number of sperm used for each insemination was adjusted based on sperm number and acrosome morphology such that each oocyte was exposed to ≥25,000 sperm with normal acrosomes/mL (58).…”

Section: Human Blood and Semen Specimensmentioning

confidence: 99%

“…Morphology assessment of fixed, unstained sperm included evaluation of sperm head size and shape and acrosome size (58).…”

Section: Human Blood and Semen Specimensmentioning

confidence: 99%

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Cadmium Concentrations in Blood and Seminal Plasma: Correlations with Sperm Number and Motility in Three Male Populations (Infertility Patients, Artificial Insemination Donors, and Unselected Volunteers)

Benoff

Hauser

Marmar

et al. 2009

Mol Med

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134

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To investigate a possible common environmental exposure that may partially explain the observed decrease in human semen quality, we correlated seminal plasma and blood cadmium levels with sperm concentration and sperm motility. We studied three separate human populations: group 1, infertility patients (Long Island, NY, USA); group 2, artificial insemination donors (AID) (Rochester, NY, USA); and group 3, general population volunteers (Rochester, NY, USA). Information about confounding factors was collected by questionnaire. Seminal plasma cadmium did not correlate with blood cadmium (Spearman correlation, n = 91, r = -0.092, P = 0.386, NS). Both blood and seminal plasma cadmium were significantly higher among infertility patients than the other subjects studied (for example, median seminal plasma cadmium was 0.282 μg/L in infertility patients versus 0.091 μg/L in AID and 0.092 μg/L in general population volunteers; Kruskal-Wallis test, P < 0.001). The percentage of motile sperm and sperm concentration correlated inversely with seminal plasma cadmium among the infertility patients (r = -0.201, P < 0.036 and r = -0.189, P < 0.05, respectively), but not in the other two groups. Age (among infertility patients) was the only positive confounder correlating with seminal plasma cadmium. To validate our human findings in an animal model, we chronically exposed adolescent male Wistar rats to low-moderate cadmium in drinking water. Though otherwise healthy, the rats exhibited decreases in epididymal sperm count and sperm motility associated with cadmium dose and time of exposure. Our human and rat study results are consistent with the hypothesis that environmental cadmium exposures may contribute significantly to reduced human male sperm concentration and sperm motility.

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“…Note that expression of all biomarkers tested correlates with the rate of fertilization in IVF (58,60,67,69).…”

Section: Sperm Function Testingmentioning

confidence: 90%