“…Studies have shown that this method is highly effective in removing bacillus spores (up to 4 log reduction) and biofilms (more than 2.2 log reduction), such as E. coli , P. fluorescens PF1, and Bacillus cereus , from stainless steel surfaces compared to standard CIP procedures (less than 2 log reduction for both, spores and biofilms) (Al Saabi et al, 2021; Dallagi, Aloui, Bouvier, et al, 2022; Dallagi, Faille, Bouvier, et al, 2022; Dallagi, Faille, Gruescu, et al, 2022). Moreover, this method has the potential to substantially reduce water and energy consumption, leading to a significant reduction in environmental impact by up to 80%, as demonstrated by life cycle assessment (Dallagi, Aloui, Bouvier, et al, 2022, Dallagi, Faille, Bouvier, et al, 2022, Dallagi, Faille, Gruescu, et al, 2022). Notably, immersing the contaminated surfaces in biosurfactant alone did not have any effect on cleaning.…”