Number of Dental Stem Cells on Root Canal Dentin after Application of Triple Antibiotic Paste or Calcium Hydroxide: An In Vitro Study

Abstract

Objective The aim of this study was to evaluate the viability of stem cells from exfoliated and deciduous teeth (SHED) on dentin surface treated with triple antibiotic paste or calcium hydroxide. Materials and Methods Nine single-rooted extracted premolars were prepared appropriately and divided into three groups. In group A, the root canals were left empty, a triple antibiotic paste was placed in the root canals of group B, and calcium hydroxide was placed in the root canals of group C. After 1 week, the intracanal medicaments were removed, and stem cells were seeded on the treated surface of the specimens for 1 more week. The cells were stained and then observed under confocal microscope over the entire surface of each test material. Counting of the cells was made by Image J (3D) software, as well as manually. Statistical Analysis To investigate any statistically significant differences between the experimental groups, statistical tests including Kruskal–Wallis and Mann–Whitney U-test were performed. Significance level was set to P < 0.05, and all analyses were performed with SPSS IBM program, v. 21. Results Groups B and C showed statistically significantly higher number of cells compared to Group A, whereas cells developed in a substrate of calcium hydroxide residues appeared in majority with distinct cores and widened unlike other groups. Conclusions The effect of calcium hydroxide manifested better results regarding the number of stems cells on root canal surfaces.

Regenerative endodontic procedure of permanent teeth with incomplete root development after application of a devitalizing agent – a case report

“…Certain conditions are required to achieve this goal: the presence of stem cells, scaffold and growth factors [11]. The vitality, proliferation and differentiation of stem cells from apical papilla (SCAP) have been shown to be easily affected by inflammation, irrigants and medications in culture [12,13]. The use of devitalizing paste in presented case might have caused harmful adverse effects on these cells.…”

Efficacy of Chitosan as Final Irrigating Solution on TGF-β1 Release from Root Canal Dentin and its Effect on Dental Pulp Stem Cells Response

“…Figure (6): Means of Optical density at 570 nm measured on micro-plate reader for different concentrations of released TGF-β1; small letters denote significant difference at P < 0.05.…”

“…Although GFs have short half-life, the binding of bioactive GFs to dentin extracellular matrix proteins protects them from biodegradation and prolongs their life-time span, which could be released in active forms during carious process or following the use of certain dental materials having the ability to cause dentin demineralization; e.g. : Ethylene diamine tetraacetic acid (EDTA), etching adhesives, and calcium hydroxide (5)(6)(7) .…”

Long-term treatment of dentine with triple antibiotic paste promotes stem cell viability and attachment

“…5 The observed variation in clinical outcomes created a critical gap regarding the effects of these medicaments on stem cell survival and attachment to the root canal of the irrigated dentine. 6 , 7 Some lines of evidence support the use of Ca(OH) 2 , 8 , 9 , 10 , 11 , although a few studies have also indicated that TAP enhanced the attachment of dental pulp stem cells (DPSCs) to the dentine. 12 These authors also found that double antibiotic paste (DAP) and Ca(OH) 2 reduced the proliferation of DPSCs.…”

“… 12 However, these findings were controversial due to the different experimental protocols used and the evaluation of stem cell survival after the short-term application of intracanal medicaments over 3- and 7-day periods. 11 , 12 , 13 …”