Number of Blastomeres in Day-2 Embryos Affect the Rates of Blastocyst Formation and Clinical Pregnancy During In Vitro Fertilization Cycles

Mi, Zuxia; Liu, Zheng; Zhu, Jing; Yao, Yufei; Zhou, Yao; Huang, Yuanhua; Qi, Li; Ma, Yanlin

doi:10.1007/s43032-021-00774-1

Cited by 3 publications

(5 citation statements)

References 19 publications

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“…The number of oocytes (11.05 ± 7.34 vs. 14.73 ± 9.15, p = .001) and high‐quality embryos (4.52 ± 3.78 vs. 6.19 ± 5.34, p = .005) on day 3 after implantation decreased considerably during ART. The definition of high‐quality embryos refers to our previously published paper 13 . Embryo quality and development were determined according to the Spanish Association of Reproduction Biology Studies (ASEBIR) criterion 14 .…”

Section: Resultsmentioning

confidence: 99%

“…The arrested embryos caused SARS‐CoV‐2 infection between Type I and Type II. In a previous study, we found that the number of blastomeres in day‐2 embryos impacts the rates of blastocyst formation and clinical pregnancy during IVF cycles 13 . It indicated that SARS‐CoV‐2 mainly reduces the quantity of high‐quality day‐3 embryos by delaying embryonic development on day 2.…”

Section: Discussionmentioning

confidence: 97%

“…In a previous study, we found that the number of blastomeres in day-2 embryos impacts the rates of blastocyst formation and clinical pregnancy during IVF cycles. 13 It indicated that SARS-CoV-2 mainly reduces the quantity of high-quality day-3 embryos by delaying embryonic development on day 2. During the IVF process, the six-cell to eight-cell stage is the key to determining the quality of embryos because the day-3 embryo with eight blastomeres is preferentially selected for fresh embryo transfer.…”

Section: Discussionmentioning

confidence: 99%

“…The definition of high-quality embryos refers to our previously published paper. 13 Embryo quality and development were determined according to the Spanish Association of Reproduction Biology Studies (ASEBIR) criterion. 14 No differences were observed concerning any of the other parameters.…”

Section: Comparison Of Cycle Characteristics and Embryo Outcomes Betw...mentioning

confidence: 99%

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SARS‐CoV‐2 infection negatively impacts on the quality of embryos by delaying early embryonic development

Ma,

Yao,

Zhang

et al. 2024

American J Rep Immunol

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BackgroundThe COVID‐19 pandemic is an unprecedented health crisis that has affected in vitro fertilization practices globally. Previous studies have shown that SARS‐CoV‐2 impacts the quality of embryos by inducing an immunological response in infertile patients. In this study, the early embryonic development of SARS‐CoV‐2‐infected infertile patients was investigated.MethodsSixty‐five SARS‐CoV‐2 infected infertile patients and 258 controls were involved in this study. The major outcome parameters for the cycle were analyzed, including the number of oocytes, maturation oocytes, available embryos per cycle, and embryo morpho kinetic characteristics.ResultsFrom SARS‐CoV‐2 infection until oocyte retrieval, it took an average of 6.63 days. The results revealed that the number of oocytes and high‐quality embryos on day 3 dramatically reduced in SARS‐CoV‐2‐infected infertile patients. SARS‐CoV‐2 was detected in the follicular fluid of three infertile patients. SARS‐CoV‐2 infection had negatively impacted the number of oocytes in multivariate linear regression models. The early embryonic development in the SARS‐CoV‐2 infection group had a noticeable delay from the six‐cell stage to blastocyst stage.ConclusionsSARS‐CoV‐2 infection reduced the number of oocytes and high‐quality embryos on day 3. It delays the early embryonic development from the six‐cell stage to blastocyst stage and has a negative impact on the quality of embryos.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Comparison Of Cycle Characteristics and Embryo Outcomes Betw...mentioning

confidence: 99%

See 2 more Smart Citations

SARS‐CoV‐2 infection negatively impacts on the quality of embryos by delaying early embryonic development

Ma,

Yao,

Zhang

et al. 2024

American J Rep Immunol

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show abstract

“…Previous studies have explored the predictive factors for embryo formation in infertile populations, but limited research has specifically addressed this issue in DOR patients, such as Mi Z et al suggesting that D2 cleavage-stage embryos with four cells have the highest rate of blastocyst formation [ 9 ]; Bassil R et al considering the diameter of the oocyte as an important factor affecting blastocyst formation, with embryos formed from oocytes measuring between 105.96 and 118.69 μm in diameter having the highest probability of forming high-quality D5 blastocysts [ 10 ]; and Yang SH et al that used time-lapse imaging systems to observe embryo morphokinetics and morphokinetic parameters, finding that the time of pronuclear fading after fertilization and the timing of blastomere division and abnormal division patterns are key factors affecting blastocyst formation [ 11 ]. However, there is a lack of research reports on the prediction of blastocyst formation combining clinical data such as patient age and endocrine status with embryo morphokinetic parameters.…”

Section: Introductionmentioning

confidence: 99%

Predictive Factors for the Formation of Viable Embryos in Subfertile Patients with Diminished Ovarian Reserve: A Clinical Prediction Study

Feng,

Zhang,

et al. 2024

Reprod. Sci.

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This study aims to construct and validate a nomogram for predicting blastocyst formation in patients with diminished ovarian reserve (DOR) during in vitro fertilization (IVF) procedures. A retrospective analysis was conducted on 445 DOR patients who underwent in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) at the Reproductive Center of Yulin Maternal and Child Health Hospital from January 2019 to January 2023. A total of 1016 embryos were cultured for blastocyst formation, of which 487 were usable blastocysts and 529 did not form usable blastocysts. The embryos were randomly divided into a training set (711 embryos) and a validation set (305 embryos). Relevant factors were initially identified through univariate logistic regression analysis based on the training set, followed by multivariate logistic regression analysis to establish a nomogram model. The prediction model was then calibrated and validated. Multivariate stepwise forward logistic regression analysis showed that female age, normal fertilization status, embryo grade on D2, and embryo grade on D3 were independent predictors of blastocyst formation in DOR patients. The Hosmer–Lemeshow test indicated no statistical difference between the predicted probabilities of blastocyst formation and actual blastocyst formation (P > 0.05). These results suggest that female age, normal fertilization status, embryo grade on D2, and embryo grade on D3 are independent predictors of blastocyst formation in DOR patients. The clinical prediction nomogram constructed from these factors has good predictive value and clinical utility and can provide a basis for clinical prognosis, intervention, and the formulation of individualized medical plans.

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Embryotoxicity evaluation of Gentamicin, an aminoglycoside antibiotic added to human embryo culture medium, using the zebrafish (Danio rerio) model

et al. 2023

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Number of Blastomeres in Day-2 Embryos Affect the Rates of Blastocyst Formation and Clinical Pregnancy During In Vitro Fertilization Cycles

Cited by 3 publications

References 19 publications

SARS‐CoV‐2 infection negatively impacts on the quality of embryos by delaying early embryonic development

SARS‐CoV‐2 infection negatively impacts on the quality of embryos by delaying early embryonic development

Predictive Factors for the Formation of Viable Embryos in Subfertile Patients with Diminished Ovarian Reserve: A Clinical Prediction Study

Embryotoxicity evaluation of Gentamicin, an aminoglycoside antibiotic added to human embryo culture medium, using the zebrafish (Danio rerio) model

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