Nullification of aspirin induced gastrotoxicity and hepatotoxicity by prior administration of wheat germ oil in Mus musculus: histopathological, ultrastructural and molecular studies

Abstract

Aspirin (acetyl salicylic acid) is used worldwide to treat various inflammatory conditions and prevent cardiovascular disease, along with reducing the risk of cancer. However, administration of aspirin causes toxic effects, especially in the stomach and liver. Thus, our study examined the protective effect of wheat germ oil on aspirin-induced toxicity in the stomach and liver tissues of Swiss albino mice. Administration of wheat germ oil before aspirin has restored normal hepatic and gastric tissue architecture and DNA integrity has become better than that of a negative health control group compared with the aspirin only treated group. The elevated gastric nitric oxide content in the aspirin only treated group was significantly decreased by wheat germ oil prior administration as a result of reduced the expression of inducible nitric synthase and increased the expression of endothelial nitric oxide synthase compared to their expression in the aspirin administered group. Wheat germ oil pre-administration significantly reduced the level of malondialdehyde, increased the level of glutathione and catalase and superoxide dismutase activities compared with those in aspirin only treated group. We conclude that wheat germ oil has a potential protective effect against aspirin induced gastro- and hepato-toxicity because of its free radical scavenging ability.

Ameliorative Impacts of Wheat Germ Oil against Ethanol-Induced Hepatic and Renal Dysfunction in Rats: Involvement of Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Signaling Pathways

“…Group I (control group): rats were given saline and unrestricted permission to water and food to evaluate the normal basic parameters. Group II, wheat germ oil group (WGO group): rats received WGO (1.5 mL/kg) (∼approximately 1.400 mg/kg) orally, every day [ 29 ] for 15 days. Group III (ethanolic group): rats were medicated with only ethanol 50% v / v (10 mL/kg) orally (about 5 g/kg), every day [ 30 ] for 15 days.…”

“…Group III (ethanolic group): rats were medicated with only ethanol 50% v / v (10 mL/kg) orally (about 5 g/kg), every day [ 30 ] for 15 days. Group IV (WGO + ethanol): rats were medicated with WGO (1.5 mL/kg) orally, every day [ 29 ] followed by ethanol 50% v / v (10 mL/kg) orally, 1 h later, every day for 15 sequential days [ 30 ]. After being anesthetized for 2 to 5 min in a desiccator with a cotton pad soaked in diethyl ether, the rats were scarified and killed by cervical dislocation.…”

Effects of taxifolin on aspirin-induced gastric damage in rats: macroscopic and biochemical evaluation

“…Aspirin is a drug that has gained worldwide acceptance in the treatment of various cardiovascular and inflammatory diseases. However, aspirin therapy causes toxic effects, especially on the stomach and liver [8]. Even low-dose aspirin administration leads to an increased risk of gastroduodenal ulcers and upper gastrointestinal bleeding [9].…”