Null allele mutants of trt-1 , the catalytic subunit of telomerase in Caenorhabditis elegans , are less sensitive to Mn-induced toxicity and DAergic degeneration

Ijomone, Omamuyovwi M.; Miah, Mahfuzur R.; Peres, Tanara Vieira; Nwoha, P.U.; Aschner, Michael

doi:10.1016/j.neuro.2016.08.016

Cited by 25 publications

(26 citation statements)

References 35 publications

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“…Several worm strains are available for visualization of DA-producing neurons in C. elegans . These include but not limited to BY200 (dat-1:GFP (vtIs1) V.) and OH7193 (otIs181 [dat-1::mCherry + ttx-3::mCherry]) III) which expresses green fluorescent protein (GFP) and mCherry respectively in their dopaminergic neurons (Caito & Aschner, 2016; Ijomone et al, 2016). Homologues of RLS genes can be modified in these worms to understand the effect of RLS genes in dopaminergic morphology.…”

Section: Elegans Methods For Rls Studymentioning

confidence: 99%

“…Worms with normal or intact DA systems down locomotion when they encounter food as opposed to worms with defective DA systems which do not slow down. Worms with deficient tyrosine hydroxylase homologue, cat-2 , are used as positive control as they show defective bacterial mechanosensation (Caito & Aschner, 2016; Ijomone et al, 2016). …”

Section: Elegans Methods For Rls Studymentioning

confidence: 99%

“…Furthermore, Fe depletion, a key feature of RLS is associated with higher deposition of Mn in the brain. There is an established C. elegans model of Mn neurotoxicity (Chen et al, 2015; Ijomone et al, 2016; Peres et al, 2018; Ruszkiewicz et al, 2018). Given the association between Mn and Fe, this model can be harnessed to evaluate whether neuronal and/or systemic alteration in Mn may contribute to the pathophysiology of RLS.…”

Section: Elegans Methods For Rls Studymentioning

confidence: 99%

“…The number of worms alive after 24 or 48 hours are counted and scored as percentage of initially transferred worms. Dose response curves are generated to determine if any genetic modifications influences survival of worms (Caito & Aschner, 2016; Ijomone et al, 2016)…”

Section: Elegans Methods For Rls Studymentioning

confidence: 99%

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Caenorhabditis elegans and its applicability to studies on restless legs syndrome

Chen

Ijomone

Lee

et al. 2019

Pharmacology of Restless Legs Syndrome (RLS)

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Restless legs syndrome (RLS) is a common neurological disorder in the US. This disorder is characterized by an irresistible urge to move the legs, although the symptoms vary in a wide range. The pathobiology of RLS has been linked to iron (Fe) deficiency and dopaminergic (DAergic) dysfunction. Several genetic factors have been reported to increase the risk of RLS. Caenorhabditis elegans (C. elegans) is a well-established animal model with a fully sequenced genome, which is highly conserved with mammals. Given the detailed knowledge of its genomic architecture, ease of genetic manipulation and conserved biosynthetic and metabolic pathways, as well as its small size, ease of maintenance, speedy generation time and large brood size, C. elegans provides numerous advantages in studying RLS-associated gene-environment interactions. Here we will review current knowledge about RLS symptoms, pathology and treatments, and discuss the application of C. elegans in RLS study, including the worm homologous genes and methods that could be performed to advance the pathophysiology RLS.

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Section: Elegans Methods For Rls Studymentioning

confidence: 99%

Section: Elegans Methods For Rls Studymentioning

confidence: 99%

Section: Elegans Methods For Rls Studymentioning

confidence: 99%

Section: Elegans Methods For Rls Studymentioning

confidence: 99%

See 2 more Smart Citations

Caenorhabditis elegans and its applicability to studies on restless legs syndrome

Chen

Ijomone

Lee

et al. 2019

Pharmacology of Restless Legs Syndrome (RLS)

Self Cite

View full text Add to dashboard Cite

show abstract

“…elegans can also be used to investigate environmental causes of ASD. For example, evidence suggest that manganese may induce ASD, and manganese exposure has been shown to cause developmental and behavioural changes in the nematodes as well (Ijomone et al, 2016). Also, neurotoxic heavy metals as potential inducers of ASD, such as lead, arsenic, and mercury have been studied extensively in worms (Du and Wang, 2009;Roh et al, 2006;Schmeisser et al, 2013;Vigneshkumar et al, 2011;Ye et al, 2010).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Worms on the spectrum - C. elegans models in autism research

Schmeißer

Parker

2018

Experimental Neurology

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Neurobehavior and neuron damage following prolonged exposure of silver nanoparticles with/without polyvinylpyrrolidone coating in Caenorhabditis elegans

Zhang

et al. 2021

J of Applied Toxicology

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Silver nanoparticles (AgNPs) have become widespread in the environment with increasing industrial applications. But the studies about their potential health risks are far from enough, especially in neurotoxic effects. This study aimed to investigate the neurotoxic effects of longer‐term exposure (prolonged exposure for 48 h and chronic exposure for 6 days) of 20nm AgNPs with/without polyvinylpyrrolidone (PVP) coating at low concentrations (0.01–10 mg·L−1) to Caenorhabditis elegans. The results suggested that exposure to AgNPs induced damage to nematode survival, with the longest and relative average life span reduced. Exposure to AgNPs caused neurotoxicity on locomotion behaviors (head thrashes, body bends, pharyngeal pumping frequency, and defecation interval) and sensory perception behaviors (chemotaxis assay and thermotaxis assay), as well as impaired dopaminergic, GABAergic, and cholinergic neurons, except for glutamatergic, based on the alters fluorescence intensity, in a dose‐ and time‐dependent manner. Further investigations suggested that the low‐dose AgNPs (0.01–0.1 mg·L−1) exposure raises receptors of GABAergic and dopamine in C. elegans at the genetic level, whereas opposite results were observed at higher doses (1–10 mg·L−1), which implied that AgNPs could cause neurotoxicity by impairing neurotransmitter delivery. The PVP‐AgNPs could cause a higher fatality rate and neurotoxicity at the same dose. Notably, AgNPs did not cause any deleterious effect on nematodes at the lowest dose of 0.01 mg·L−1. In general, these results suggested that AgNPs possess the neurotoxic potential in C. elegans and provided useful information to understand the neurotoxicity of AgNPs, which would offer an inspiring perspective on the safe application.

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Null allele mutants of trt-1 , the catalytic subunit of telomerase in Caenorhabditis elegans , are less sensitive to Mn-induced toxicity and DAergic degeneration

Cited by 25 publications

References 35 publications

Caenorhabditis elegans and its applicability to studies on restless legs syndrome

Caenorhabditis elegans and its applicability to studies on restless legs syndrome

Worms on the spectrum - C. elegans models in autism research

Neurobehavior and neuron damage following prolonged exposure of silver nanoparticles with/without polyvinylpyrrolidone coating in Caenorhabditis elegans

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