Nuisance species in lake constance revealed through eDNA

Abstract

Biological invasions are a global threat to biodiversity especially for aquatic resources. The distribution of alien species is associated with human activities; therefore, exotic species tend to accumulate near big urban areas through different invasion vectors such as ballast water, hull fouling, aquarium and pet releases. The Rhine River region is one of the most economically important in Europe. Around 60 million people live in the river basin that is connected with other large European rivers via the Rhine-Main-Danube shipping canal. The Alpine Rhine flows to Lake Constance, which is the second largest subalpine lake in Europe. Here, eDNA metabarcoding was employed to inventory aquatic species from water samples in six riverine and four lake localities within Lake Constance region. A 313 bp fragment within cytochrome c oxidase subunit I gene was PCR amplified using generalist primers for metazoan and sequenced with MiSeq High-Throughput Sequencing platform. Seven invertebrate invasive species and the invasive fish Oncorhynchus mykiss were detected from eDNA. Species-specific primers were employed to confirm metabarcoded species. Most of the invasive species detected in this study correspond to samples from areas around lake ports, followed by other lake and degraded downstream river areas. Samples taken upstream of Lake Constance were free of invertebrate aliens. To establish common regulation and management actions regarding aquatic invasions in the three countries that share Lake Constance is recommended.

Development of an eDNA‐based survey method for urban fish markets

“…turbidity or the amount of water that can be collected). While our study focused on the use of eDNA for monitoring a market area, the success we had in retrieval, amplification and sequencing of eDNA from the urban environment supports the use of this technique in monitoring species/taxa in other urban contexts, such as invasive fish or amphibian species in riverine port areas and urban water bodies (Charvoz et al, 2021;Clusa et al, 2021).…”

River benthic macroinvertebrates and environmental DNA metabarcoding: a scoping review of eDNA sampling, extraction, amplification and sequencing methods

“…Since eDNA is mostly captured from river water samples, another aspect to be considered during sampling is the volume of the water that needs to be filtered to efficiently represent the local macroinvertebrate communities. One liter is a commonly used volume of water filtered for the assessment of river macroinvertebrates through eDNA metabarcoding (Fernández et al 2018(Fernández et al , 2019Macher et al 2018;Bagley et al 2019;Hajibabaei et al 2019;Uchida et al 2020;Clusa et al 2021;Hupalo et al 2022;Reinholdt et al 2021;Seymour et al 2021). Machler et al (2016 who sampled different volumes of river water ranging from 0.25 to 2 L and studied the effect of sampled water volume on the detection of three macroinvertebrate species, suggest a minimum water volume of 1 L to reduce false negative detections.…”

Environmental DNA survey indicates arrival of quagga mussel in Ticino River basin

“…As a result, molecular surveys based on environmental DNA (eDNA) are increasingly used for the early detection of invasive species, because they provide a highly sensitive, cost-effective, and non-invasive approach (Morisette et al, 2021). These have been successfully applied for the surveillance of many freshwater invasive species, including the quagga mussel (De Ventura et al, 2017;Sepulveda et al, 2019;Blackman et al, 2020;Clusa et al, 2021).…”