Nudeotide sequence of the transforming early region Elb of adenovirus type 12 DNA: structure and gene organization, and comparison with those of adenovirus type 5 DNA

Abstract

The nucleotide sequence of the entire transforming early region of E1b of the highly oncogenic adenovirus type 12 (Ad12) DNA has been determined. The total sequence (3860 base pairs) encompasses the entire transforming early region E1 of Ad12 DNA. From the sequence for the E1b region of Ad12, and the transcription map of the E1b region (1, 2, 3, and this paper) the structure and gene organization of the early region E1b of Ad12 DNA were analyzed and compared with those of the E1b region in the non-oncogenic Ad5 DNA (4, 5). Most of the sequences in the E1b region of Ad12 was highly homologous to that of Ad5. It is predicted that the Ad12 region E1b codes for polypeptides of 53.9, 19.1, and 8.9 kd. This situation is identical with that of the Ad5 region E1b which codes for polypeptides of 54.9, 20.6, and 8.3 kd. The function of these predicted polypeptides encoded by the E1b regions in cell transformation is discussed.

The adenovirus type 12 early-region 1B 58,000-Mr gene product is required for viral DNA synthesis and for initiation of cell transformation

“…The adenovirus type S (Ad5) E1B gene codes for 21,000-(21K) and 55,000-molecular-weight polypeptides (9), the Ad2 E1B gene codes for 19,000-and 53,000-molecular-weight polypeptides (1,43) and the Adl2 E1B gene codes for 19,000 and 58,000-molecular-weight polypeptides (9,26,36,44). The Ad5 or Ad2 E1B 19K polypeptide is important for maintenance of cell transformation (2, 11) and for protection of cellular and viral DNA degradation (31,43,47).…”

Structure of viral DNA in a rat cell line transformed by the clonedEcoRI-Cfragment of adenovirus 12

“…4. Comparison of the deduced sequences with those in the corresponding regions of Adl2 DNA (19,20) indicated that these two fragments indeed contained the viral-nonviral junctions. As indicated in Fig.3, the left-hand junction was mapped at position 118 and the right-hand junction at position 3520, respectively.…”

Hyperproduction of adenovirus type 12 E1B gene product in monkey cells, using a simian virus 40 vector

“…j, and k). The 50 K protein, encoded in a different reading frame and synthesized from the second AUG of the 2.2-kilobase E1B mRNA (2,9), could not be detected in the recombinantinfected cells. The results indicate that the material revealed by immunofluorescence consists of the 19K protein and that the 19K protein tends to form a condensed structure in the cytoplasm when its concentration is high enough.…”

“…The ElB gene encodes at least two proteins, with molecular weights of 19,000 to 21,000 (19K to 21K) and 50K to 55K (4,11,13,16,18). Recent sequence data on the AdS and Adl2 E1B regions have shown that there are three open frames for protein synthesis (2,9). In the case of Adl2, the first reading frame starts from ATG 1,541 and ends at TAA 2,030 and is capable of coding for the 19K protein.…”