Nude mice are nonpermissive towards the anti‐dextran response of congenic cells

Abstract

Nude mice bearing allotype Ighb on a BALB/c genetic background (= CB nu/nu) are nonresponders to alpha (1----3)dextran (Dex), in contrast to BALB/c or BALB/c nu/nu. Although CB nu/nu mice accept transplants of congenic BALB/c, or BALB nu/nu lymphocytes, as shown by the expression of donor allotype Igha, they are not permissive for a primary anti-Dex response by the grafted cells. BALB/c or BALB nu/nu cells, however, give a strong anti-Dex response when grafted onto irradiated CB nu/nu or CB 23 (Ighb) euthymic mice. A thymus-independent, radiation-sensitive suppressor cell population is postulated, which specifically hinders the anti-Dex response, and which is exhibited by strains bearing that portion of chromosome 12 which codes for CH allotype Ighb, not containing the germ-line anti-Dex V/D genes. The suppressive action of Ighb lymphocytes could be demonstrated directly in staggered co-transfer experiments.

The Transplantation Barrier of Nude Mice

“…Other data from our group demonstrate that the barrier in nude mice does not result in elimination of transferred donor cells but leads to persistence in the nude host (6,26).…”

“…It has been suggested that this transplantation barrier in non-irradiated animals towards thymus-dependent memory cells is due to a thymus-dependent suppression or rejection (5). But it has been shown recently that also nude mice can be nonpermissive towards thymus-independent congeneic immune cells (6). From recent experiments, we conclude that the «isogeneic barrief» is linked to the differentiation stage of B cells in the recipient (7), since B cell-defective xidmice are permissive towards trans plan ted lymphocytes.…”

Effects of IgM Allotype Suppression on Serum IgMLevels, B‐1 and B‐2 Cells, and Antibody Responses irAllotype Heterozygous F1 Mice

“…Mice that received bone marrow as a source of progenitors for B-2 B cells were unable to respond to DEX. Additionally, other previous studies (Weiler et al, 1985) demonstrated active suppression of the DEX-specific Igh6a Ab response by Igh6b lymphocytes when BALB/c bone marrow was transferred into nude mice expressing the Igh6b allotype on a BALB/c genetic background. It is likely that the demonstrated suppressive action of host Igh6b lymphocytes would explain the lack of donor Igh6a DEX-specific Ab response in the mice receiving bone marrow cells in the cell-transfer experiments (FOrster and Rajewsky, 1987) similar to that observed by Weiler and colleagues (1985).…”