Nucleotide variants of the Nramp1 gene in pigs and their effects on disease resistance

Wen, Yuliang; Cheng, Xiao Wen; Zhao, Shengguo

doi:10.18805/ijar.v0iof.8491

Cited by 4 publications

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“…AA and AB were linked to mastitis of which AB was found with higher frequency in affected animals. Similar study by Wen et al (2018) reported that differences in the disease resistance of the Tibetan and Yorkshire pig breeds was due to nucleotide differences in transmembrane domains and tertiary structures of NRAMP protein. However, no study have been reported to explore variation in transcription factor binding sites (TFBS) and its possible association with disease in Indian buffalo TNF-α gene.…”

Section: Introductionsupporting

confidence: 55%

Detection of polymorphism in the promoter region of TNF-alpha gene of water buffalo (Bubalus bubalis) and its association with disease resistance

Kumar

Mishra

Lavakumar

et al. 2019

IJAR

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Tumour necrosis factor (TNF), a proinflammatory cytokine, plays an important role in the regulation of cell differentiation, proliferation and death, as well as in innate and adaptive immune response. In this study we have sequence characterized upstream regulatory region of TNF-á gene of buffalo. Ten single polymorphic nucleotides (SNPs) -541A/T, -553A/G, -563,C/G, -619A/G, -659A/G, -706C/G, -730G/T, -794A/G, -898G/T, -981A/G) along with one indel at position -783 were identified. Further when analysed, among riverine and swamp types, 16 and 17 transcription factor–binding sites (TFBS) respectively were observed. Riverine and swamp buffaloes differed by PAX-2 transcription factor binding site at position -541 present in riverine and absent in swamp, important in the proliferation of multiple cell lines and development of organs. Comparative analysis of TNF-á upstream region revealed presence of additional NF-k binding site in buffalo and v-Myb binding site in cattle only. Genotyping of SNP -794A/G by tetra-ARMS PCR revealed variable allele frequencies among riverine and swamp buffaloes, however in clinical mastitis affected and non-affected Murrah buffaloes this SNP didn’t show any significant association with mastitis resistance. Overall study reveals the sequence variation in TNF-á promoter, possibly leading to modification of transcriptional regulation in swamp and riverine buffalo.

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Section: Introductionsupporting

confidence: 55%

Detection of polymorphism in the promoter region of TNF-alpha gene of water buffalo (Bubalus bubalis) and its association with disease resistance

Kumar

Mishra

Lavakumar

et al. 2019

IJAR

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“…There is a large number of studies researching the effect of SLC11A1 and FUT1 genes polymorphism on animal immunity (Dai et al, 2017;Liu et al, 2017;Prajapati et al, 2017, Bosewell et al, 2018Wen et al, 2018;Ardiyana et al, 2020). It is well known that pigs with better disease resistance may have better growth, greater weight and better feed conversion (Tuggle et al, 2005), but the question of the relationship between the polymorphism of these genes and pig productivity has not been studied enough, the studies are fragmentary.…”

Section: Discussionmentioning

confidence: 99%

Association of Fut1 and Slc11a1 gene polymorphisms with productivity traits of Large White pigs

Sukhno

Vashchenko

Saenko

et al. 2022

Regul. Mech. Biosyst.

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The purpose of our work was to study the polymorphism of genes associated with disease resistance and to search for their associations with productive traits in the population of the Ukrainian Large White pigs. For this study, 50 pigs were used, observations and measurements were carried out at the age from birth to 180 days. Genetic studies were carried out in a certified laboratory of the Institute of Pig Breeding and Agroindustrial Production. In the study of fucosyltransferase 1 and solute carrier family 11 member 1 genes, polymorphism was found in three of the five analyzed loci. In the Ukrainian Large White subpopulation of pigs the informativeness of these gene polymorphisms was at the optimal level for associative analysis, Polymorphism Information Content was greater than 0.3 in two loci. A sufficiently high level of Polymorphism Information Content indicates the value of this breed to preserve the biodiversity of pigs. The distribution of genotypes at some loci of the solute carrier family 11 member 1 gene was characterized by a deviation from the theoretically expected one due to the increase in the frequency of the heterozygous genotype. There was also a statistically confirmed deviation of the genotypes’ distribution from the normal and polymorphism fucosyltransferase 1 gene, but in this case in the direction of increasing the frequency of both homozygous variants. These results indicate the presence of a certain selection pressure on the mentioned polymorphisms and their possible impact on productive traits. The influence of solute carrier family 11 member 1 gene polymorphism on the weight of pigs at the age of 120 and 180 days, the average daily gain recorded in the period 28–120 days and from birth to 180 days, as well as on the backfat thickness, was established. The preferred genotype is TT, which can be used in breeding to obtain more productive animals with increased disease resistance, but in the selection of animals at this locus, it is necessary to control the backfat thickness and prevent breeding of pigs that may worsen this trait.

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“…Domestic and foreign research found that the Nramp1 genes in cattle, sheep, rabbits, chickens and mice are related to disease resistance (Qin et al, 2013;Qiu et al, 2015;Liu et al, 2017). Wen et al (2018) studied the structure and sequence of Nramp1 in Tibetan, Gansu Black, Large White, Yorkshire and Duroc pigs. The sequence analysis of the Nramp1 gene in these five pig breeds revealed 11 nucleotide variants in the intronic regions, 2 nucleotide variants in the control region, 10 nucleotide variants and one deletion in the 3 non-coding region and 15 nucleotide variants in the exons (Wen et al, 2018).…”

Section: Correlation Analysis Between Nramp1 Gene and Piglet Diarrheamentioning

confidence: 99%

“…Wen et al (2018) studied the structure and sequence of Nramp1 in Tibetan, Gansu Black, Large White, Yorkshire and Duroc pigs. The sequence analysis of the Nramp1 gene in these five pig breeds revealed 11 nucleotide variants in the intronic regions, 2 nucleotide variants in the control region, 10 nucleotide variants and one deletion in the 3 non-coding region and 15 nucleotide variants in the exons (Wen et al, 2018). In the present study, PCR-RFLP was used to detect the polymorphisms in the exon 2 of the Nramp1 gene in four breeding pigs.…”

Section: Correlation Analysis Between Nramp1 Gene and Piglet Diarrheamentioning

confidence: 99%

Polymorphism of Nramp1 Gene and Its Association with Diarrhea in Pigs

Chen

Peng

et al. 2020

IJAR

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Background: Natural resistance associated macrophage protein 1 (Nramp1) is a relatively conservative gene that plays a crucial role in swine immune response and disease resistance. Methods: We identified the polymorphisms and gene variations in the exon 2 of Nramp1 using polymerase chain reaction–restriction fragment length polymorphism and investigated the correlation between the polymorphisms and piglet diarrhea in four pig breeds (Bamei, Duroc, Landrace, and Large White pigs). Result: The results showed that two alleles (A and B) were identified in all the pig breeds, three genotypes (AA, BB, and AB) were detected in Bamei and Large White breeds, and two genotypes (AA and AB) were detected in Landrace and Duroc breeds. Allele A and genotype AB were dominant in Bamei, Large White, and Landrace breeds, whereas genotype AA was dominant in Duroc pigs. A moderate polymorphism was observed in Landrace and Large White pigs, and polymorphism was abundant in Bamei pigs and low in Duroc pigs. The Chi-square test for Hardy–Weinberg equilibrium disclosed that the exon 2 of Nramp1 in the four breeds of pigs did not deviate from the Hardy–Weinberg balance (P less than 0.05). The results of association analysis showed a significant correlation between breed and piglet diarrhea (P less than 0.05), and the diarrhea score of Bamei pigs was much lower than those of the other breeds. The study could supply theoretical references for further functional research on Nramp1 gene and for screening genes related to disease-resistance breeding.

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Nucleotide variants of the Nramp1 gene in pigs and their effects on disease resistance

Cited by 4 publications

References 0 publications

Detection of polymorphism in the promoter region of TNF-alpha gene of water buffalo (Bubalus bubalis) and its association with disease resistance

Detection of polymorphism in the promoter region of TNF-alpha gene of water buffalo (Bubalus bubalis) and its association with disease resistance

Association of Fut1 and Slc11a1 gene polymorphisms with productivity traits of Large White pigs

Polymorphism of Nramp1 Gene and Its Association with Diarrhea in Pigs

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