Nucleotide-specific PCR for molecular virus typing

Marschall, Manfred; Schuler, Anke; Böswald, Christoph; Helten, Anke; Hechtfischer, Anne; Lapatschek, Matthias; Meier-Ewert, Herbert

doi:10.1016/0166-0934(94)00159-e

Cited by 6 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Modern PCR approaches are central to the characterisation of influenza and other viruses at the molecular genetic level and have been exploited in this role for several decades. [7][8][9] This is achieved through the initial extraction of viral DNA or RNA, the removal of non-nucleic acid material, the use of reverse transcriptase where necessary to generate single stranded cDNA, the design and use of primers that anneal to predetermined target sequences, amplication of those targets with or without chain termination, and the detection of the amplified products by gel electrophoresis or chromatography aided by the use of fluorescent or radioactive labelled nucleic acid bases. Typing of subtyping of virus strains uses primers specific to various human and avian influenza strains and can be performed on virus isolates or directly on clinical specimens.…”

Section: Surveillance Of the Influenza Viruscurrent Non-molecular And...mentioning

confidence: 99%

Proteotyping for the rapid identification of influenza virus and other biopathogens

Downard

2013

Chem. Soc. Rev.

View full text Add to dashboard Cite

The influenza virus is one of the most deadly infectious agents known to man and has been responsible for the deaths of some hundred million lives throughout human history. The need to rapidly and reliably survey circulating virus strains down to the molecular level is ever present. This tutorial describes the development and application of a new proteotyping approach that harnesses the power of high resolution of mass spectrometry to characterise the influenza virus, and by extension other bacterial and viral pathogens. The approach is shown to be able to type, subtype, and determine the lineage of human influenza virus strains through the detection of one or more signature peptide ions in the mass spectrum of whole virus digests. Pandemic strains can be similarly distinguished from seasonal ones, and new computer algorithms have been written to allow reassorted strains that pose the greatest pandemic risk to be rapidly identified from such datasets. The broader application of the approach is further demonstrated here for the parainfluenza virus, a virus which can be life threatening to children and presents similar clinical symptoms to influenza.

show abstract

Section: Surveillance Of the Influenza Viruscurrent Non-molecular And...mentioning

confidence: 99%

Proteotyping for the rapid identification of influenza virus and other biopathogens

Downard

2013

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

“…More recently, nucleotide-specific PGR was shown to discriminate between two variants of influenza virus C [20]. Primers have been shown to discriminate between the PI and P4 isolates of pea seedbome mosaic virus [14,15].…”

Section: Chapter 1 Use Of Unique Rna Sequence-specific Oligonucleotimentioning

confidence: 99%

Molecular basis of resistance to soybean mosaic virus: reverse transcription (RT)-PCR and strain complementation analysis in soybeans

Omunyin

View full text Add to dashboard Cite

The inqxjrtance of soybeans 1 Potyvirus characteristics and gencxue organization Hist(xy and econoniic importance of soybean mosaic virus Taxonomy Characteristics of SMV Diagnostic techniques SMV strains Virus transmission Host resistance to virus disease Rsv gene resistance to SMV Virus movement in plants and the phenomenon of complementation Polymerase chain reaction and disease detection Dissertaticm organization 31 Literature cited 32 CHAPTER L USE OF UNIQUE RNA SEQLfiiNCE-SPECinC OLIGONUCLEOTIDE

show abstract

The ORF, Regulated Synthesis, and Persistence-Specific Variation of Influenza C Viral NS1 Protein

Marschall¹,

Helten²,

Hechtfischer³

et al. 1999

Virology

View full text Add to dashboard Cite

The open reading frame (ORF) and the regulated synthesis of the influenza C viral NS1 protein were analyzed in view of viruses possessing different biological activities. We provide evidence for a 246-amino-acid NS1-ORF, encoded by five viral strains and variants. Prokaryotic expression of the prototype NS1-ORF resulted in a product of 27 kDa, confirming the predicted molecular weight. Using an antiserum raised against recombinant NS1 protein, nonstructural proteins of wild-type virus were detected in infected cells for a limited course of time, whereas a persistent virus variant was characterized by a long-term nonstructural gene expression. As examined by infection experiments, the intracellular distribution of nonstructural protein was nuclear and cytoplasmic, whereas in NS1 gene-transfected cells, the cytoplasmic localization occurred in a fine-grained structure, suggesting an analogy to influenza A viral NS1 protein. Concerning persistent infection, NS1 protein species differing in sizes and posttranslational modifications were observed for a persistent virus variant, as particularly illustrated by a high degree of NS1 phosphorylation. Virus reassortant analyses proved the importance of the NS-coding genomic segment: the minimal viral properties required for the establishment of persistence were transferred with this segment to a monoreassortant virus. Thus the influenza C viral NS1 protein is a 246-amino-acid nuclear-cytoplasmic phosphoprotein that can be subject to specific variations being functionally linked to a persistent virus phenotype.

show abstract

Nucleotide-specific PCR for molecular virus typing

Cited by 6 publications

References 12 publications

Proteotyping for the rapid identification of influenza virus and other biopathogens

Proteotyping for the rapid identification of influenza virus and other biopathogens

Molecular basis of resistance to soybean mosaic virus: reverse transcription (RT)-PCR and strain complementation analysis in soybeans

The ORF, Regulated Synthesis, and Persistence-Specific Variation of Influenza C Viral NS1 Protein

Contact Info

Product

Resources

About