Nucleotide sequences of theatpB and theatpE genes of the brown algaPylaiella littoralis (L.) Kjellm

Abstract

The atpB and the atpE genes encode for the/~ and subunits of the proton-translocation ATPase complex in prokaryotes, mitochondria and chloroplasts. In E. coli and e-purple eubacteria the genes for all subunits of the ATPase complex are linked and constitute a single transcriptional unit [1,2]. In contrast, the cyanobacterial and green plastid atpB and atpE genes form a separate 'atpB' cluster which is encoded by the chloroplastic genome in plants [3,4].Little is known about the organization of AT-Pase genes in brown and red algae. In this report we present the nucleotide sequence of the atpB-atpE region encoded by the chloroplastic genome of the brown alga PylaieIla littoralis (L.) Kjellm (Fig. 1).The 4.2 kb Eco RI chloroplastic DNA fragment E13 of P. littoralis [5] hybridized with a spinach atpB probe [6] and was sequenced in both directions using the dideoxy sequencing method and synthetic oligonucleotides (Eurogentec S.A., Libge, Belgium). This region contains two adjacent open reading frames, which by comparison with other known sequences have been identified as the atpB and atpE genes. Their lengths are 1446 bp (482 codons) and 399 bp (133 codons) respectively. They are separated by a 14 nucleotide spacer region (Fig. 1).Comparisons with homologous genes from prokaryotes and green plastids (Figs. 2 and 3) indicate that the atpB genes are, in all cases, more conserved than the atpE genes. The percentages of identical amino acid residues between the atpB and atpE genes of P. littoralis and those of other species (calculated from the alignments, data not shown) indicate that these genes probably have a cyanobacterial origin as do those of green plastids [5].Because of the similar organisation of this region to that of other plants it appears probable that these genes are also cotranscribed [4]. Figure 4 shows the similarities in the organization of the junction region between the atpB and atpE genes of land plants and P. littoralis. It is noteworthy that the AAATGA sequence, which in land plants comprises the atpB stop codon and the atpE start codon (implying a slide backwards of ribosomes to the -1 frame on mRNA) is exactly conserved at the same position in P. littora-Iis, although the putative atpB stop codon is 12The nucleotide sequence data reported will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases underthe accession number X60329.

Organization of plastid-encoded ATPase genes and flanking regions including homologues of infB and tsf in the thermophilic red alga Galdieria sulphuraria

“…Kowallik, personal communication). Additionally, the sequence of the atpB/E cluster from D. dichotoma [29] and Pylaiella littoralis [23] were published. Interestingly, the arrangement of atpl in the multicellular red alga Antithamnion sp.…”

Molecular phylogeny of theatpB andatpE genes of the brown algaPylaiella littoralis

“…Aligned amino acid sequences for the atpB and atpE genes were taken from Jouannic et al (1992). Corresponding codon insertions were introduced into the nucleotide sequences.…”

“…In the plastid genome of the brown alga Pylaiella ]ittoralis (L.) Kjellm., these two genes are organised in a cluster and separated by a I4-nucleotide spacer region (Jouannic et al, 1992).…”

An improved method of protein isolation and proteome analysis with Saccharina japonica (Laminariales) incubated under different pH conditions

“…3) was also separated. This ATPase α/β chains family protein is a subunit of the catalytic core of Ftype ATPase and is related to ATP generation (Jouannic et al 1992). Fucoxanthin-chlorophyll a-c binding protein and ATP synthase subunit β are involved in photosynthesis (Apt et al 1995).…”