Nucleotide sequences of segments 1, 3 and 4 of the genome of Bombyx mori cypovirus 1 encoding putative capsid proteins VP1, VP3 and VP4, respectively

Hagiwara, Kyoji; Rao, S. Venkat; Scott, S. W.; Carner, G. R.

doi:10.1099/0022-1317-83-6-1477

Cited by 57 publications

(29 citation statements)

References 16 publications

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“…Genomic dsRNA of UsCPV was extracted from purified polyhedra by using a QIAampViral minikit from Qiagen (13,24). The RNA was precipitated, washed with 75% ethanol, dried under a vacuum, and resuspended in RNase-free water.…”

Section: Methodsmentioning

confidence: 99%

Morphological and Molecular Characterization of a Cypovirus ( Reoviridae ) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae)

Shapiro

Green

Rao

et al. 2005

J Virol

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A novel cypovirus has been isolated from the mosquito Uranotaenia sapphirina (UsCPV) and shown to cause a chronic infection confined to the cytoplasm of epithelial cells of the gastric ceca and posterior stomach. The production of large numbers of virions and inclusion bodies and their arrangement into paracrystalline arrays gives the gut of infected insects a distinctive blue iridescence. The virions, which were examined by electron microscopy, are icosahedral (55 to 65 nm in diameter) with a central core that is surrounded by a single capsid layer. They are usually packaged individually within cubic inclusion bodies (polyhedra, ϳ100 nm across), although two to eight virus particles were sometimes occluded together. The virus was experimentally transmitted per os to several mosquito species. The transmission rate was enhanced by the presence of magnesium ions but was inhibited by calcium ions. Most of the infected larvae survived to adulthood, and the adults retained the infection. Electrophoretic analysis of the UsCPV genome segments (using 1% agarose gels) generated a migration pattern (electropherotype) that is different from those of the 16 Cypovirus species already recognized. UsCPV genome segment 10 (Seg-10) showed no significant nucleotide sequence similarity to the corresponding segment of the other cypoviruses that have previously been analyzed, and it has different "conserved" termini. A BLAST search of the UsCPV deduced amino acid sequence also showed little similarity to Antheraea mylitta CPV-4 (67 of 290 [23%]) or Choristoneura fumiferana CPV-16 (33 of 111 [29%]). We conclude that UsCPV should be recognized as a member of a new Cypovirus species (Cypovirus 17, strain UsCPV-17).

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Section: Methodsmentioning

confidence: 99%

Morphological and Molecular Characterization of a Cypovirus ( Reoviridae ) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae)

Shapiro

Green

Rao

et al. 2005

J Virol

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“…The molecular weight of the P3 protein of RRSV (131K) is similar to that of the major capsid shell VP1 protein of CPV (148K) and the 1 protein of Orthoreovirus (142K). The P3 protein of RRSV has partial homologies to the major capsid shell VP1 protein of CPV (12,13), which has conserved holding and protein organization among capsid shell proteins of the reoviruses (37,42). Indeed, the secondarystructure prediction showed that the P3 protein of RRSV contains many ␣-helical elements in addition to the ␤-strand elements, which suggests that the P3 protein of RRSV has ␣ ϩ ␤ holds, a structure similar to those of other members of Reoviridae (3).…”

Section: Resultsmentioning

confidence: 99%

“…The turrets of RRSV are larger than those of Orthoreovirus (28) and CPV (approximately 150 Å in diameter and 100 Å in height in both cases) (42). RRSV contains at least six structural proteins, namely P1, P2, P3, P4A, P8B, and P9, with molecular weights of 138,000 (138K), 133K, 131K, 141K, 42K, and 39K, respectively (13,15,(32)(33)(34), that are encoded by 10 segments of the dsRNA genome (26). The capsid of RRSV, encapsidating RdRp (P4A protein) (33) and 10 segments of dsRNA, appears to consist of at least four kinds of protein: P2, a capping enzyme (31); P3, a capsid shell protein (13); P8, a major capsid protein that is cleaved to yield P8A and P8B (32); and P9, a spike protein involved in transmission via the insect vector (6a, 34, 41).…”

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confidence: 99%

Structural Evolution of Reoviridae Revealed by Oryzavirus in Acquiring the Second Capsid Shell

Miyazaki

Uehara-Ichiki

et al. 2008

J Virol

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The conservation of the core structure and diversification of the external features among the turreted reoviruses appear to be relevant to structural evolution in facilitating the infection of diverse host species. The structure of Rice ragged stunt virus (RRSV), in the genus Oryzavirus of the family Reoviridae, is determined to show a core composed of capsid shell, clamps, and long turrets. The RRSV core structure is equivalent to the core structure of Orthoreovirus and the virion structure of Cytoplasmic polyhedrosis virus (CPV). In RRSV, five peripheral trimers surround each long turret and sit at the Q trimer position in the T‫31؍‬l icosahedral symmetry, a structural feature unique to turreted reoviruses. That is, the core of RRSV is partially covered by 60 copies of the peripheral trimer. In contrast, the core of Orthoreovirus is covered by 200 copies of the trimer that sit at the Q, R, S, and T trimer positions. Our results suggest that among the three viruses, RRSV has a structure intermediate between that of Orthoreovirus and the CPV virion. This conclusion coincides with the results of the phylogenetic analysis of amino acid sequences of RNA-dependent RNA polymerases.Reoviridae is the largest and most diverse family of doublestranded RNA (dsRNA) viruses. It includes 12 established genera, namely Aquareovirus, Coltivirus, Cypovirus, Fijivirus, Idnoreovirus, Mycoreovirus, Orbivirus, Orthoreovirus, Oryzavirus, Phytoreovirus, Rotavirus, and Seadornavirus. The hosts of these viruses include plants, vertebrates, insects, and fungi (22). All known viruses in this family are 600 to 800 Å in diameter and consist of an inner core that is surrounded by a few layers of protein, with the exception of the single-layered Cytoplasmic polyhedrosis virus (CPV), which encapsidates 9 to 12 segments of dsRNA and the enzymes involved in transcription. Whereas the precise morphology varies among genera, the morphologies of the innermost capsid shells are similar in spite of the absence of significant sequence homology among component proteins. The conserved innermost capsid of reoviruses is composed of 120 copies of thin crescent-shaped proteins, and the respective subunits exhibit similar overall folding, with substantial modifications that appear to have developed during viral evolution (11,24,28,37,42). Except in CPV, the innermost capsid shell is covered by additional outer layers, the organization and structure of which vary among the genera in the family. The outer capsid shell appears to play important roles in maintaining the stability of the thin innermost capsid shell and sequestering the dsRNA genome, as well as in conferring host specificity and mediating entry into host cells.Reoviruses can be divided structurally into two subgroups, the turreted and nonturreted reoviruses, on the basis of a critical structural feature. Members of the seven genera Aquareovirus, Cypovirus, Fijivirus, Idnoreovirus, Mycoreovirus, Orthoreovirus, and Oryzavirus are classified as turreted reoviruses (22), with distinctive pentameric tu...

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“…However, information regarding the genomic makeup of CPVs is rather limited. The complete nucleotide sequences for only three CPV types (1, 14, and 15) have been reported and deposited into GenBank [9,[11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

The complete nucleotide sequence of the type 5 Helicoverpa armigera cytoplasmic polyhedrosis virus genome

Tan

Zhang

et al. 2008

Virus Genes

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The S1-6, S8, and S9 segments of the type 5 Helicoverpa armigera cytoplasmic polyhedrosis virus (HaCPV-5, Chinese strain) were cloned and sequenced, completing the HaCPV-5 genome. We found that each HaCPV-5 segment exhibits the conserved terminal sequences AGUU and UUGC located at the 5' and 3' ends, respectively. We also analyzed the translation initiation codon of the HaCPV-5 genome and compared it with the available cypovirus sequences in GenBank. We postulated that the conserved purine at the -3 position in relation to the AUG codon is probably the most important nucleotide for efficient translation initiation in cypovirus. Although the nucleotide sequences of the HaCPV-5 segments S1-10 exhibit no significant similarity to other viruses, blast searches did reveal some similarities between predicted HaCPV-5 amino acid sequences and those of other viruses.

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Nucleotide sequences of segments 1, 3 and 4 of the genome of Bombyx mori cypovirus 1 encoding putative capsid proteins VP1, VP3 and VP4, respectively

Cited by 57 publications

References 16 publications

Morphological and Molecular Characterization of a Cypovirus ( Reoviridae ) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae)

Morphological and Molecular Characterization of a Cypovirus ( Reoviridae ) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae)

Structural Evolution of Reoviridae Revealed by Oryzavirus in Acquiring the Second Capsid Shell

The complete nucleotide sequence of the type 5 Helicoverpa armigera cytoplasmic polyhedrosis virus genome

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