Nucleotide sequence predicts circularity and self-cleavage of 300-ribonucleotide satellite of arabis mosaic virus

Biologically Active Transcripts of a Large Satellite RNA from Arabis Mosaic Nepovirus and the Importance of 5' End Sequences for its Replication

“…The hop isolate of ArMV used contains a 300 nucleotide RNA satellite (Davies & Clark, 1983;Kaper et al, 1988), but the ArMV isolates from ivy, ash or sugar-beet do not contain extragenomic RNAs (Liu et al, 1991;unpublished data). When M t R transcribed from 2~tg of linearized template D N A was inoculated with RNA-1 and RNA-2 from the ivy or ash isolates of ArMV, satellite RN A was detected (by slot blot hybridization) in extracts from the tip leaves of C. quinoa plants and was also found to be encapsidated in tests done after the second passage cycle (Fig.…”

The ubiquitous hammerhead ribozyme

“…Two different types of subviral plant pathogens harbor HHRs, viroids, and plant virus satellite RNAs, both of which are circular, single-stranded RNA molecules of z250-400 nt. Viroids are infectious by themselves (Flores et al 2004;Tabler and Tsagris 2004), while plant virus satellite RNAs depend in their propagation on their cognate virus and modulate the virus' symptoms Kaper et al 1988;Rubino et al 1990). These two types of subviral plant pathogens are thought to replicate in a rolling circle mechanism (Branch and Robertson 1984), which can proceed in either a symmetric or asymmetric manner (Flores et al 2004;Tabler and Tsagris 2004).…”

The nucleotide sequence of a satellite RNA associated with strawberry latent ringspot virus

“…The DOT PLOT, PILEUP and CLUSTAL comparison program (Staden, 1984(Staden, , 1986 and GCG search programs (e.g. BLAST, FASTA, T-FASTA, WORD-SEARCH; Devereux et al, 1984) did not reveal any significant homology (except at the 5' end), either at the nucleic or amino acid level, between the SLRSV satRNA sequence and satRNAs from the following: TBRV (Meyer et al, 1984), ArMV-L (Liu et al, 1990), grapevine fan leaf nepovirus (Fuchs et al, 1989), chicory yellow mottle nepovirus (Rubino et al, 1990), or the type D satRNAs from ArMV (Kaper et al, 1988), or tobacco ringspot nepovirus (Buzayan et al, 1986) or the cucumber mosaic cucumovirus (type C; Collmer et al, 1983). We have determined the complete nucleotide sequence in cDNA clones representing the RNA 2 of SLRSV-H.…”