Nucleotide sequence of the β-lactamase I gene of<i>Bacillus cereus</i>strains 569/H and 5/B

Madonna, M J; Zhu, Ying Fang; Lampen, J. O.

doi:10.1093/nar/15.4.1877

Cited by 16 publications

(7 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies had shown that B. cereus harbors the antibiotic resistance genes encoding β-lactamases [ 11 , 23 , 48 , 49 ]. Therefore, B. cereus has the ability to degrade β-lactams and show resistant characteristics [ 23 , 28 , 29 , 48 , 49 ]. In contrast, B. cereus isolated from edible fungi were still sensitive to aminoglycosides, ketolide, glycopeptides, quinolones, and phenylpropanol antibiotics, in agreement with some previous studies [ 24 , 27 – 29 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we found that B. cereus isolates from edible fungi were mainly resistant to β-lactams (except cefotetan and imipenem) and ansamycins, especially ampicillin and penicillin. Previous studies had shown that B. cereus harbors the antibiotic resistance genes encoding β-lactamases [11,23,48,49]. Therefore, B. cereus has the ability to degrade β-lactams and show resistant characteristics [23,28,29,48,49].…”

Section: Multi-locus Sequence Typing and Clustering Of The Isolatesmentioning

confidence: 99%

See 1 more Smart Citation

Assessment and molecular characterization of Bacillus cereus isolated from edible fungi in China

Liu

et al. 2020

BMC Microbiol

View full text Add to dashboard Cite

Background Bacillus cereus is a foodborne pathogen commonly found in nature and food and can cause food spoilage and health issues. Although the prevalence of B. cereus in foods has been reported worldwide, the extent of contamination in edible fungi, which has become increasingly popular as traditional or functional food, is largely unknown. Here we investigated the prevalence, toxin genes’ distribution, antibiotic resistance, and genetic diversity of B. cereus isolated from edible fungi in China. Results Six hundred and ninety-nine edible fungi samples were collected across China, with 198 (28.3%) samples found to be contaminated by B. cereus, with an average contamination level of 55.4 most probable number (MPN)/g. Two hundred and forty-seven B. cereus strains were isolated from the contaminated samples. Seven enterotoxin genes and one cereulide synthetase gene were detected. The detection frequencies of all enterotoxin genes were ≥ 80%, whereas the positive rate of the cesB gene in B. cereus was 3%. Most isolates were resistant to penicillins, β-lactam/β-lactamase inhibitor combinations, cephems, and ansamycins, but were susceptible to penems, aminoglycosides, macrolides, ketolide, glycopeptides, quinolones, phenylpropanol, tetracyclines, lincosamides, streptogramins, and nitrofurans. Meanwhile, 99.6% of all isolates displayed multiple antimicrobial resistance to three or more classes of antimicrobials. Using genetic diversity analysis, all isolates were defined in 171 sequence types (STs), of which 83 isolates were assigned to 78 new STs. Conclusions This study provides large-scale insight into the prevalence and potential risk of B. cereus in edible fungi in China. Approximately one-third of the samples were contaminated with B. cereus, and almost all isolates showed multiple antimicrobial resistance. Detection frequencies of all seven enterotoxin genes were equal to or more than 80%. These new findings may indicate a need for proper pre-/post-processing of edible fungi to eliminate B. cereus, thereby preventing the potential risk to public health.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Multi-locus Sequence Typing and Clustering Of The Isolatesmentioning

confidence: 99%

Assessment and molecular characterization of Bacillus cereus isolated from edible fungi in China

Liu

et al. 2020

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…They possess a C-terminal penicillin-binding domain that catalyses the penicillin-sensitive peptidoglycan transpeptidase reaction and an N-terminal domain that is assumed to catalyse the penicillin-insensitive peptido- Table 1. Origin and main characteristics (primary and tertiary structures) of the enzymes studied Key to reference numbers cited in the Table below: (1) Neugebauer et al (1981); (2) Sloma & Gross (1983); Madonna et al (1987); (3) Sutcliffe (1978); (4) Wang & Novick (1987); (5) Dehottay et al (1987); (6) Jaurin & Grundstrom (1981); (7) Lindberg & Normark (1986); (8) Dale et al (1985); (9) Duez et al (1987); (10) Broome-Smith et al (1983); (11) Todd et al (1986); (12) Broome-Smith et al (1985a); (13) Asoh et al (1986); (14) Nakamura et al (1983); (15) Kelly et al (1986); (16) Samraoui et al (1986); (17) Knox et al (1976); (18) Herzberg & Moult (1987); (19) Dideberg et al (1987); (20) Kelly et al (1987).…”

Section: Materials and Methods Enzymesmentioning

confidence: 99%

The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 dd-peptidase family

Joris

Ghuysen

Dive

et al. 1988

Biochemical Journal

344

276

View full text Add to dashboard Cite

Homology searches and amino acid alignments, using the Streptomyces R61 DD-peptidase/penicillin-binding protein as reference, have been applied to the beta-lactamases of classes A and C, the Oxa-2 beta-lactamase (considered as the first known member of an additional class D), the low-Mr DD-peptidases/penicillin-binding proteins (protein no. 5 of Escherichia coli and Bacillus subtilis) and penicillin-binding domains of the high-Mr penicillin-binding proteins (PBP1A, PBP1B, PBP2 and PBP3 of E. coli). Though the evolutionary distance may vary considerably, all these penicillin-interactive proteins and domains appear to be members of a single superfamily of active-site-serine enzymes distinct from the classical trypsin or subtilisin families. The amino acid alignments reveal several conserved boxes that consist of strict identities or homologous amino acids. The significance of these boxes is highlighted by the known results of X-ray crystallography, chemical derivatization and site-directed-mutagenesis experiments.

show abstract

“…Strain ATCC 14579 is the type strain (20). Strain ATCC 10876 is also called B. cereus 569 (2), and this strain and its derivative have been widely used for the study of ,B-lactamases (1,13,14). Strain ATCC 11778 has been used as a test organism for tetracycline antibiotics (3).…”

Section: Methodsmentioning

confidence: 99%

Physical maps of the genomes of three Bacillus cereus strains

Carlson¹,

Grønstad²,

Kolstø³

1992

J Bacteriol

View full text Add to dashboard Cite

NotI restriction maps of the chromosomes from Bacillus cereus ATCC 10876, ATCC 11778, and the B. cereus type strain ATCC 14579 have been established and compared with the previously established map of B. cereus ATCC 10987. Between 10 and 14 NotI fragments were observed, ranging from 15 to 1,300 kb, in digests of DNA from the various strains. The sizes of the genomes varied between 5.4 and 6.3 Mb. The maps were constructed by hybridization of 42 random probes, prepared from B. cereus ATCC 10987 libraries, to fragments from partial and complete NotI digests, separated by pulsed-field gel electrophoresis. Nine probes were specific for ATCC 10987 only. Probes for five B. subtilis and five B. cereus genes were also used. The NotI restriction fragment patterns of the four strains were strikingly different.

show abstract

Nucleotide sequence of the β-lactamase I gene ofBacillus cereusstrains 569/H and 5/B

Cited by 16 publications

References 3 publications

Assessment and molecular characterization of Bacillus cereus isolated from edible fungi in China

Assessment and molecular characterization of Bacillus cereus isolated from edible fungi in China

The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 dd-peptidase family

Physical maps of the genomes of three Bacillus cereus strains

Contact Info

Product

Resources

About