Nucleotide sequence of the Thiobacillus ferrooxidans chromosomal gene encoding mercuric reductase

Mercury Pollution and Bioremediation—A Case Study on Biosorption by a Mercury-Resistant Marine Bacterium

“…They vary in number and identity of genes involved, and are encoded by the mer operon located on plasmids (Summers and Silver, 1978;Brown et al, 1986;Griffin et al, 1987;Radstrom et al, 1994;Osborn et al, 1997), chromosomes (Wang et al, 1987(Wang et al, , 1989Inoue et al, 1989Inoue et al, , 1991Iohara et al, 2001), transposons Kholodi et al, 1993;Liebert et al, 1997Liebert et al, , 1999Hobman et al, 1994), and integrons (Liebert et al, 1999). Gram-negative bacterial mer operons have been studied more extensively than those of Gram-positive bacteria although Gram-positive bacteria have the same set of genes arranged in a similar order.…”

Volatilization of Mercury by an Iron Oxidation Enzyme System in a Highly Mercury-resistantAcidithiobacillus ferrooxidansStrain MON-1

“…13,[17][18][19][20] A. ferrooxidans cells have mercury reductase activity 6,9,21,22) and the genes involved in the volatilization of mercury have been cloned and characterized in detail. 6,[23][24][25][26][27] A. ferrooxidans SUG 2-2 was isolated as a mercury-resistant strain among one hundred A. ferrooxidans strains isolated from natural environments. 10) Strain SUG 2-2 has an ability to volatilize metal mercury from mercury-polluted wastewater and soil under acidic conditions in the presence of ferrous iron.…”

Genome wide identification of Acidithiobacillus ferrooxidans (ATCC 23270) transcription factors and comparative analysis of ArsR and MerR metal regulators

“…In fact, studies of MerD from Ralstonia metallidurans indicated that MerD was able to displace Hg-bound MerR from the mer operator allowing the synthesis of metal-free MerR in order to shut down the induction of the mer genes when the external mercury is exhausted (Champier et al 2004). Previous studies have determined the composition of chromosomal mer operon of A. ferrooxidans strain E-15 (Inoue et al 1991;Inoue et al 1989;Rawlings and Kusano 1994), it consists of a functional promoter region followed by merR (the regulatory gene), merC (encoding a mercury transport protein), and merA (encoding the enzyme mercuric reductase) (Inoue et al 1996;Kusano et al 1990;Inoue et al 1991;Dietrich et al 2008), however no orthologs of merD were observed in the mer operon of this strain. Similarly, no merD gene was present in the putative mer operons of A. ferrooxidans strain ATCC 53993 (NC_011206), which contains orthologs of merR (Lferr_0159 and Lferr_0164) and merC (Lferr_0160 and Lferr_0163) genes.…”