Nucleotide sequence of the phage T5 DNA segment containing six tRNA genes

Abstract

Here, we present the nucleotide sequence of the bacteriophage T5 EcoRl/HpaI DNA fragment (27.8%-29.5% Rhoades' map (1)). The fragment contains six tRNA genes. Earlier corresponding transfer RNAs were identified by Hunt et al. (2), and tRNAAs was sequenced (3). Except tRNA genes four ORFs were found, and three of them have suitable RBS which are located on the proper distance from the initiation codons. The promoter sequences upstream the genes for tRNATYr and ORF 42aa were characterized by the cloning in the specialized promoter-probe plasmid pKWIII (the data will be published elsewhere). EMBL accession no. Z14121 Noncoding (RNA-like) strand is presented in the direction 5'-3' from the HpaI site. This sequence is immediately adjacent to the distal part of T5 tRNA gene region we sequenced earlier (4).